Monitoring mitochondrial viscosity with anticancer phosphorescent Ir(iii) complexes via two-photon lifetime imaging

Chemical Science
Liang HaoZong-Wan Mao

Abstract

Precise quantitative measurement of viscosity at the subcellular level presents great challenges. Two-photon phosphorescence lifetime imaging microscopy (TPPLIM) can reflect micro-environmental changes of a chromophore in a quantitative manner. Phosphorescent iridium complexes are potential TPPLIM probes due to their rich photophysical properties including environment-sensitive long-lifetime emission and high two-photon absorption (TPA) properties. In this work, a series of iridium(iii) complexes containing rotatable groups are developed as mitochondria-targeting anticancer agents and quantitative viscosity probes. Among them, Ir6 ([Ir(ppy-CHO)2(dppe)]PF6; ppy-CHO: 4-(2-pyridyl)benzaldehyde; dppe: cis-1,2-bis(diphenylphosphino)ethene) shows satisfactory TPA properties and long lifetimes (up to 1 μs). The emission intensities and lifetimes of Ir6 are viscosity-dependent, which is mainly attributed to the configurational changes in the diphosphine ligand as proved by 1H NMR spectra. Ir6 displays potent cytotoxicity, and mechanism investigations show that it can accumulate in mitochondria and induce apoptotic cell death. Moreover, Ir6 can induce mitochondrial dysfunction and monitor the changes in mitochondrial viscosity simultane...Continue Reading

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Citations

Nov 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuanyan LiWai-Yeung Wong
Mar 4, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Wei-Wei QinLiang He
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Apr 2, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yi LiZong-Wan Mao
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Jan 7, 2022·Dalton Transactions : an International Journal of Inorganic Chemistry·Yun-Qiong GuHong Liang

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Methods Mentioned

BETA
biosensing
phosphorescence
fluorescence lifetime imaging
phosphorescence lifetime
X-ray
column chromatography
NMR
flow cytometry
confocal microscopy

Software Mentioned

TPPLIM

Related Concepts

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