Monitoring neovascularization and integration of decellularized human scaffolds using photoacoustic imaging.

Photoacoustics
Olumide OgunladePaul Beard

Abstract

Tissue engineering is a branch of regenerative medicine that aims to manipulate cells and scaffolds to create bioartificial tissues and organs for patients. A major challenge lies in monitoring the blood supply to the new tissue following transplantation: the integration and neovascularization of scaffolds in vivo is critical to their functionality. Photoacoustic imaging (PAI) is a laser-generated ultrasound-based technique that is particularly well suited to visualising microvasculature due to the high optical absorption of haemoglobin. Here, we describe an early proof-of-concept study in which PAI in widefield tomography mode is used to image biological, decellularized human tracheal scaffolds. We found that PAI allowed the longitudinal tracking of scaffold integration into subcutaneous murine tissue with high spatial resolution at depth over an extended period of time. The results of the study were consistent with post-imaging histological analyses, demonstrating that PAI can be used to non-invasively monitor the extent of vascularization in biological tissue-engineered scaffolds. We propose that this technique may be a valuable tool for studies designed to test interventions aimed at improving the speed and extent of scaffo...Continue Reading

Citations

Jul 2, 2020·Journal of Biomedical Materials Research. Part a·Elahe Masaeli, Mohammad Hossein Nasr-Esfahani
Aug 17, 2019·Tissue Engineering. Part C, Methods·Yunhao ZhuXueding Wang
May 2, 2020·Cell and Tissue Research·Reinhard Pabst
Dec 20, 2019·Tissue Engineering. Part B, Reviews·Binita ShresthaEric M Brey
Mar 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Parinaz AbdollahiyanAhad Mokhtarzadeh
Jun 12, 2021·Regenerative Medicine·Fei SunHongcan Shi
Jul 15, 2021·Advanced Science·Johannes ReblingDaniel Razansky
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fei SunHongcan Shi

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Methods Mentioned

BETA
imaging techniques
irradiate

Software Mentioned

Amira
PAM
FEI Visualization Sciences
MATLAB
Wave
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