Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay.

Virologica Sinica
Tingting NingYouchun Wang

Abstract

The Hantaan virus (HTNV) and Seoul virus (SEOV) mutants have accumulated over time. It is important to determine whether their neutralizing epitopes have evolved, thereby making the current vaccine powerless. However, it is impossible to determine by using traditional plaque reduction neutralization test (PRNT), because it requires large numbers of live mutant strains. Pseudovirus-based neutralization assays (PBNA) were developed by employing vesicular stomatitis virus (VSV) backbone incorporated with HTNV or SEOV glycoproteins (VSVΔG*-HTNVG or VSVΔG*-SEOVG). 56 and 51 single amino acid substitutions of glycoprotein (GP) in HTNV and SEOV were selected and introduced into the reference plasmid. Then the mutant pseudoviruses were generated and tested by PBNA. The PBNA results were highly correlated with PRNT ones with R2 being 0.91 for VSVΔG*-HTNVG and 0.82 for VSVΔG*-SEOVG. 53 HTNV mutant pseudoviruses and 46 SEOV mutants were successfully generated. Importantly, by using PBNA, we found that HTNV or SEOV immunized antisera could neutralize all the corresponding 53 HTNV mutants or the 46 SEOV mutants respectively. The novel PBNA enables us to closely monitor the effectiveness of vaccines against large numbers of evolving HTNV and...Continue Reading

References

Jan 1, 1989·Archives of Virology·X K ZhangN Hashimoto
Apr 1, 1997·Emerging Infectious Diseases·C Schmaljohn, B Hjelle
Feb 9, 2000·Trends in Microbiology·B J Meyer, C S Schmaljohn
Feb 24, 2000·Journal of Medical Virology·C de Carvalho NicacioE Björling
Jul 6, 2000·Journal of Medical Microbiology·C McCaughey, C A Hart
Apr 23, 2003·Virology·Joachim KochEkkehard K F Bautz
Dec 27, 2005·Current Molecular Medicine·Svetlana F KhaiboullinaStephen C St Jeor
Jan 1, 2008·Journal of Infection in Developing Countries·Zhenqiang BiCathy E Roth
Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Apr 22, 2011·Human Vaccines·Detlev H KrügerBoris Klempa
Apr 24, 2013·Critical Reviews in Microbiology·Dionysios Christos WatsonGeorge Panos
Sep 11, 2013·Nature Reviews. Microbiology·Antti VaheriJukka Mustonen
Jul 12, 2014·BMC Infectious Diseases·Shuo ZhangDexin Li
Dec 8, 2015·Frontiers in Microbiology·Musalwa MuyangwaAlbert A Rizvanov
Jan 23, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Lu-Xi ZouLing Sun
Jun 30, 2016·Cell Reports·Sai LiThomas A Bowden
Oct 27, 2016·PLoS Pathogens·Shmuel WillenskyMoshe Dessau
Jan 22, 2019·Archives of Virology·Piet MaesJens H Kuhn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.