Monitoring of an Alkaline 2,4,6-Trichlorophenol-Degrading Enrichment Culture by DNA Fingerprinting Methods and Isolation of the Responsible Organism, Haloalkaliphilic Nocardioides sp. Strain M6.

Applied and Environmental Microbiology
O Maltseva, P Oriel

Abstract

A site situated near Alkali Lake (Oregon) and highly contaminated by chloroaromatic compounds was chosen for isolation of alkaliphilic chlorophenol-degrading bacteria. Prolonged cultivation of an enrichment culture followed by successive transfers resulted in a strong increase in the 2,4,6-trichlorophenol (2,4,6-TCP) degradation rate. Repetitive extragenic palindromic PCR and amplified ribosomal DNA restriction analysis were applied to distinguish members of the enrichment culture and monitor them during the enrichment procedure. Comparison of the fingerprints of the isolates obtained from the enrichment culture and its total DNA fingerprint indicated the presence of an unidentified bacterium in the enrichment culture, assisting in its isolation. The 2,4,6-TCP-degrading isolate, M6, was tentatively identified as a Nocardioides sp. strain based on its partial 16S RNA sequence and fatty acid profile. Strain M6 was capable of utilizing up to 1.6 g of 2,4,6-TCP per liter as a sole carbon and energy source and could also grow on 2,4-dichlorophenol and 2,4,5-trichlorophenol. A high-cell-density suspension of this strain degraded a wide range of chlorinated phenols from di- to pentachlorophenol while showing a clear preference for phe...Continue Reading

References

Jan 1, 1991·Biodegradation·L A GolovlevaS Kuzmin
Jul 1, 1991·Applied and Environmental Microbiology·D Y LiF Lingens
Dec 1, 1982·Applied and Environmental Microbiology·G J Stanlake, R K Finn
May 1, 1996·Microbiology·Olga MaltsevaPatrick Oriel
Jul 1, 1997·Applied and Environmental Microbiology·V G MathesonL J Forney

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Citations

Dec 15, 2020·Journal of Environmental Health Science & Engineering·Mehdi HassanshahianMahla Askarinejad Behzadi
Jan 30, 1999·Applied and Environmental Microbiology·T TangheW Verstraete
Jun 20, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Mohd Wajid Ali Khan, Masood Ahmad
Oct 7, 2015·Frontiers in Microbiology·L Shivlata, Tulasi Satyanarayana
Jul 14, 2001·FEMS Microbiology Ecology·A G. HayG S. Sayler
Nov 6, 2014·Canadian Journal of Microbiology·Baisuo ZhaoShulin Chen
Aug 22, 2007·Applied Microbiology and Biotechnology·H Al-AwadhiS S Radwan
Sep 11, 2012·Journal of Basic Microbiology·Santosh Kr Karn, M Sudhakara Reddy
Jun 28, 2016·World Journal of Microbiology & Biotechnology·Mohamed Faraj EdbeibFahrul Huyop

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