PMID: 8606789Nov 1, 1995Paper

Monitoring of black widow spider venom (BWSV) induced exo- and endocytosis in living frog motor nerve terminals with FM1-43

Neuropharmacology
A W Henkel, W J Betz

Abstract

The neurotoxin Black Widow Spider Venom (BWSV) triggers massive release of neurotransmitter at synapses. Here we demonstrate that the action of BWSV on the frog neuromuscular junction can be visualized in vivo by the use of the fluorescent styryl dye FM1-43. This vital dye stains recycled synaptic vesicles upon nerve stimulation. Motor nerve terminals were stained with FM1-43 via electrical stimulation, washed and then exposed to BWSV or alpha-Latrotoxin. All terminals destained completely, independent of external calcium. Exposure of frog nerve terminals to BWSV in the presence of FM1-43 and calcium led to staining of terminals. The staining pattern appeared to be exactly the same as in control preparations, stimulated electrically via the nerve. When the same experiment was performed in the absence of calcium, only a minute quantity of dye was taken up into the nerve terminals, and the synapses looked swollen and puffed. Addition of external calcium to these preparations elicited an immediate shrinking of the nerve terminals, indicating endocytosis. These observations support electron-microscopic data that suggest an important role for extracellular calcium in endocytosis of BWSV poisoned nerve terminals.

References

Jan 14, 1986·Biochemical and Biophysical Research Communications·E WankeJ Meldolesi
Oct 1, 1980·The Journal of Cell Biology·B Ceccarelli, W P Hurlbut
Jul 1, 1984·The Journal of Cell Biology·F ValtortaB Ceccarelli
Mar 1, 1994·The Journal of Cell Biology·W J Betz, A W Henkel
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Rosenboom, M Lindau

❮ Previous
Next ❯

Citations

Nov 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Lachlan D Rash, Wayne C Hodgson
Jun 1, 1996·Current Opinion in Neurobiology·W J BetzC B Smith
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Annette DenkerSilvio O Rizzoli
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·A W HenkelW J Betz
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M A RahmanY A Ushkaryov
Jun 19, 2003·The Journal of Physiology·Haibing Teng, Robert S Wilkinson
Apr 15, 1999·Annual Review of Neuroscience·A J CochillaW J Betz
Aug 25, 2001·Annual Review of Neuroscience·T C Südhof
Jul 10, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ferenc DeákThomas C Südhof
Sep 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tao SunLing-Gang Wu
Nov 28, 2013·Annual Review of Physiology·Ling-Gang WuHsueh-Cheng Chiang
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S S VogelN A Lambert
Aug 31, 2007·Neurochemistry International·A S TarasenkoN H Himmelreich
Oct 1, 1996·Molecular Membrane Biology·S J McClure, P J Robinson
Jul 21, 2010·Biophysical Journal·Dirk KaminSilvio O Rizzoli
Mar 21, 1998·Neuroscience·H C Palfrey, C R Artalejo
Sep 4, 2007·Trends in Neurosciences·Liming He, Ling-Gang Wu
Feb 10, 2011·Nature Reviews. Neuroscience·Volker HauckeStephan J Sigrist
Dec 3, 1999·Journal of Neurochemistry·M A Cousin, P J Robinson
Apr 4, 2000·Physiological Reviews·G SchiavoC Montecucco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.