Monitoring of blood oxygenation in brain by resonance Raman spectroscopy

Journal of Biophotonics
Nadezda A BrazheOlga Sosnovtseva

Abstract

Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm-1 (symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm-1 and 1602 and 1638 cm-1 (vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries. in vivo measurements of blood oxygenation in the cortex of mice ventilated with inspiratory gas mixtures containing different amounts of oxygen-normoxia, hyperoxia and hypoxia-validate the proposed approach. Our method allows to visualize blood saturation with O2 in different microvascular networks.

References

Aug 18, 1976·Journal of the American Chemical Society·T KitagawaM I Saito
Nov 1, 1977·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·F F JöbsisM Rosenthal
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D KleinfeldW Denk
Feb 1, 2002·Nature Medicine·Hayrunnisa BolayMichael A Moskowitz
Feb 15, 2003·Science·Jeffrey K ThompsonRalph D Freeman
Apr 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Masahito NemotoArthur W Toga
Jul 9, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Cenk AyataMichael A Moskowitz
Mar 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ivo P Torres FilhoKevin R Ward
Apr 20, 2005·Pathophysiology : the Official Journal of the International Society for Pathophysiology·O V RodnenkovE I Chazov
Mar 8, 2006·Critical Care Medicine·Kevin R WardJames Terner
Dec 8, 2006·Analytical and Bioanalytical Chemistry·Bayden R WoodDon McNaughton
Feb 15, 2007·Analytical Chemistry·Kevin R WardJames Terner
Apr 4, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·O G LunevaE I Chazov
Oct 30, 2007·Nature Neuroscience·Costantino Iadecola, Maiken Nedergaard
Jan 1, 2008·Journal of Biomedical Optics·Martin WolfValentina Quaresima
Mar 29, 2008·Journal of Applied Physiology·Ivo P Torres FilhoKevin R Ward
Jun 13, 2008·Nature·Nikos K Logothetis
Dec 17, 2009·Biophysical Journal·Nadezda A BrazheGeorgy V Maksimov
Nov 12, 2010·Nature·David AttwellEric A Newman
Jan 18, 2011·Nature Medicine·Robert P J BarrettoMark J Schnitzer
Jul 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Clotilde LecruxEdith Hamel
Aug 23, 2011·Epilepsy Research·Theodore H SchwartzChristian-G Bénar
Oct 19, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Rickson C MesquitaArjun G Yodh
Nov 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sune N Jespersen, Leif Østergaard
Jan 19, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna DevorDavid A Boas
Dec 6, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Claus MathiesenMartin Lauritzen
Apr 30, 2013·Biochimica Et Biophysica Acta·Angela FagoCelia Bonaventura
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Barbara Lykke LindMartin J Lauritzen
Jan 1, 2014·The Journal of Trauma and Acute Care Surgery·Mohamad H TibaKevin R Ward
Mar 29, 2014·Nature·Catherine N HallDavid Attwell
Mar 30, 2016·The Journal of Surgical Research·Ivo Torres FilhoKevin R Ward

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Citations

Aug 14, 2019·Journal of Biophotonics·Rong ShenDegui Wang
Jun 1, 2020·Journal of Biophotonics·Georgette Azemtsop MatanfackJürgen Popp

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