PMID: 11324973Apr 28, 2001Paper

Monitoring of cell and tissue responses to photodynamic therapy by electrical impedance spectroscopy

Physics in Medicine and Biology
A Molckovsky, B C Wilson

Abstract

Electrical impedance spectroscopic (EIS) monitoring of photodynamic therapy (PDT) was investigated in vivo in rat liver and in vitro in multicellular spheroids. Liver impedance was continuously measured with two needle electrodes before, during and up to 3 hours following Photofrin-PDT. EIS spectra were altered immediately after PDT, with significant changes in conductivity at approximately 10 kHz, and in permittivity at approximately 30 kHz and 1 MHz. The change in permittivity at high frequencies was related to oedema, while low-frequency effects were attributed to cell necrosis and vascular changes. Photofrin-PDT-treated spheroids showed dose-dependent decreases in permittivity and conductivity at frequencies above 10 and 100 kHz, respectively. Histology showed concomitant development of a damaged rim containing sparsely distributed cells with compromised membranes and lightly staining cytoplasm. Different EIS responses to apoptotic versus necrotic modes of cell death further verified the sensitivity of impedance to purely cellular changes in the spheroid model. In conclusion, EIS sensitivity to PDT-induced damage, at both the cell and tissue level, varies with dose and time, and can be correlated qualitatively to biological...Continue Reading

References

Nov 1, 1992·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·A SurowiecZ Petrovich
Jan 1, 1992·Photochemistry and Photobiology·B W Henderson, T J Dougherty
May 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J W DobruckiH M Swartz
Apr 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·M S PattersonB C Wilson
Nov 1, 1990·Physics in Medicine and Biology·L A Dissado
Mar 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T L CecklerR G Bryant
Aug 1, 1989·British Journal of Cancer·N J DoddB Wood
Nov 1, 1987·Photochemistry and Photobiology·W P JeevesP Spiegl
May 1, 1986·IEEE Transactions on Bio-medical Engineering·S R SmithG L Wolf
Jan 15, 1987·American Journal of Ophthalmology·A A HidayatN A Rao
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck
May 1, 1994·Annals of Biomedical Engineering·P Héroux, M Bourdages
May 1, 1994·Physiological Measurement·B RigaudJ P Morucci
Jan 1, 1997·Photochemistry and Photobiology·I GeorgakoudiT H Foster
Jun 26, 1998·Journal of Photochemistry and Photobiology. B, Biology·B W McIlroyA J MacRobert
Jul 29, 1998·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J NuutinenR Kolle
Jan 30, 1999·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·F StewartW Star
Mar 31, 1999·International Journal of Radiation Oncology, Biology, Physics·D A McRaeS C Mueller
May 27, 1999·Skin Pharmacology and Applied Skin Physiology·V C ColussiH Mukhtar
Jun 18, 1999·Annals of the New York Academy of Sciences·O CasasJ Cinca
Jun 18, 1999·Annals of the New York Academy of Sciences·M SchäferM M Gebhard
Jun 18, 1999·Annals of the New York Academy of Sciences·I Nicander, S Ollmar
Dec 1, 1994·Physics in Medicine and Biology·M G Nichols, T H Foster
Oct 1, 1997·Lasers in Medical Science·B C WilsonL Lilge

❮ Previous
Next ❯

Citations

Mar 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alyssa MasterAnirban Sen Gupta
Jul 23, 2008·Technology in Cancer Research & Treatment·Zheng HuangYang K Chen
Apr 11, 2008·Physics in Medicine and Biology·Brian C Wilson, Michael S Patterson
Jan 31, 2018·ACS Chemical Biology·Mario M ModenaAndreas Hierlemann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.