Monitoring of cell oxygenation and responses to metabolic stimulation by intracellular oxygen sensing technique

Integrative Biology : Quantitative Biosciences From Nano to Macro
Alexander ZhdanovDmitri Papkovsky

Abstract

Quenched-phosphorescence oxygen (O(2)) sensing technique allows non-invasive, real-time monitoring of both intra- and extracellular O(2) concentration in respiring samples. Using this technique we investigated O(2) gradients in populations of neurosecretory PC12 cells cultured in 96-well plates and exposed to graded hypoxia at rest and upon metabolic stimulation. Under high atmospheric O(2) (10-21%) the respiration of resting cells dictated that local O(2) was moderately reduced, and at a certain threshold (6% in galactose medium) cell layer became practically anoxic. Furthermore, cell stimulation triggered a major redistribution of O(2) and a prominent 'hypoxic overshoot' mediated by diffusion. The deep, prolonged cell deoxygenation upon stimulation was matched by an increase in nuclear HIF-1alpha levels. In the presence of nitric oxide the hypoxic overshoot was truncated and HIF-1alpha stabilization inhibited. Thus, the main determinants which impact upon cellular O(2) levels and oxygen-sensitive signaling pathways are the atmospheric O(2), sample geometry, cell density, respiration rate and its dynamics. Changes in any of these parameters can significantly alter the O(2) levels experienced by the cells and the subsequently a...Continue Reading

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Citations

Sep 8, 2010·Cellular and Molecular Life Sciences : CMLS·Alexander V ZhdanovDmitri B Papkovsky
Jan 18, 2012·Cellular and Molecular Life Sciences : CMLS·Ruslan I Dmitriev, Dmitri B Papkovsky
Sep 11, 2013·Biomaterials·Ruslan I DmitrievDmitri B Papkovsky
Oct 24, 2012·Biochemistry Research International·Thilo Hagen
Mar 7, 2013·Biochimica Et Biophysica Acta·Alexander V ZhdanovDmitri B Papkovsky
Feb 6, 2015·Acta Biomaterialia·James JenkinsDmitri B Papkovsky
Sep 19, 2014·Analytical Biochemistry·Alina V KondrashinaDmitri B Papkovsky
Dec 3, 2014·Experimental Cell Research·Alexander V ZhdanovDmitri B Papkovsky
Jul 10, 2014·Cellular and Molecular Life Sciences : CMLS·Ruslan I DmitrievDmitri B Papkovsky
Aug 20, 2016·Stem Cells and Development·Abhilasha TiwariMark A Kirkland
Sep 6, 2018·American Journal of Physiology. Cell Physiology·Ming YaoMichael P Gamcsik
Sep 17, 2010·American Journal of Physiology. Cell Physiology·Eiji Takahashi, Michihiko Sato
Aug 12, 2016·Cellular and Molecular Life Sciences : CMLS·Alexander V ZhdanovDmitri B Papkovsky
Jun 19, 2016·The Biochemical Journal·James JenkinsRuslan I Dmitriev
Apr 21, 2015·Methods and Applications in Fluorescence·Ruslan I Dmitriev, Dmitri B Papkovsky
Jun 19, 2013·Chemical Society Reviews·Dmitri B Papkovsky, Ruslan I Dmitriev
May 16, 2018·Cellular and Molecular Life Sciences : CMLS·Dmitri B Papkovsky, Ruslan I Dmitriev
Oct 10, 2018·Journal of Biomedical Optics·Rozhin PenjweiniJay R Knutson
Oct 27, 2018·Oxidative Medicine and Cellular Longevity·Jeffrey A StuartLucas A Maddalena
Mar 11, 2020·Frontiers in Endocrinology·Jiri Pavlacky, Jan Polak
Jun 7, 2014·Biomaterials Science·Ruslan I DmitrievDmitri B Papkovsky
Aug 8, 2015·American Journal of Physiology. Cell Physiology·A V ZhdanovD B Papkovsky
Jun 27, 2019·Proceedings. Mathematical, Physical, and Engineering Sciences·Disha B Sheth, Miklόs Gratzl
Feb 6, 2020·Biomedical Optics Express·Rajannya SenDmitri Papkovsky
Mar 3, 2012·Analytical Chemistry·Ruslan I DmitrievDmitri B Papkovsky
Aug 31, 2021·The Journal of Biological Chemistry·Cameron A SchmidtP Darrell Neufer

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