Monitoring of CO2 and O2 concentrations in the headspace of Sakaguchi flasks during liquid culture of microorganism

Applied Microbiology and Biotechnology
Masato Takahashi, Hideki Aoyagi

Abstract

CO2 and O2 in the Sakaguchi flask headspace during culture was monitored via circulation direct monitoring and sampling system (CDMSS), a device with circulation bypass system. In static culture with Saccharomyces cerevisiae (circulation rate, 50 mL/min), a vertical CO2 concentration gradient (maximum gap ~ 2% (v/v) [height from the bottom of flask 45 mm, 7%; 155 mm, 5%]) in the Sakaguchi flask headspace was observed; no concentration O2 gradient was observed. However, shake flask culture showed vertical gradient concentrations for both CO2 and O2 (maximum gap of CO2 and O2 concentrations: 2 and 4% [heights from the bottom of flask 115 mm, 6.0 and 9.5%; 175 mm, 4.0 and 13.5%], respectively). When the CDMSS circulation rate in the Sakaguchi flask headspace was 300 or 400 mL/min, the gaseous environment was uniformly distributed so that no vertical gradient concentration was observed. In shaking culture with Escherichia coli under these conditions, CO2 was accumulated at high concentrations in the headspace and culture broth (maximum values 8%, in the headspace; 120 mg/L, in the culture broth). Most of the accumulated CO2 in the headspace could be removed by inserting a column packed with CO2 adsorbent at the bypass port of the C...Continue Reading

References

Aug 1, 1989·The Journal of Applied Bacteriology·N M Dixon, D B Kell
Feb 15, 2001·Biochemical Engineering Journal·T Anderlei, J Büchs
Aug 25, 2009·Bioprocess and Biosystems Engineering·Konstantin SchneiderElmar Heinzle
Nov 8, 2014·Microbial Cell Factories·Sylvia DiederichsJochen Büchs
Mar 28, 2018·Applied Microbiology and Biotechnology·Masato Takahashi, Hideki Aoyagi

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