Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose

Journal of Oral Microbiology
Irene DigeSebastian Schlafer

Abstract

pH in dental biofilms is of central importance for the development of caries. We used the ratiometric pH-sensitive dye C-SNARF-4 in combination with digital image analysis to monitor extracellular pH in dental biofilms grown in situ with and without sucrose supply. Dental biofilms (48 h) from 10 individuals were collected on glass slabs mounted on intra-oral appliances. During growth, appliances were immersed extra-orally in either physiological saline or 4% sucrose for 2 min, eight times per day. Fluorescence emissions of C-SNARF-4 in deep layers of the biofilms were recorded ex vivo with confocal microscopy for 15 min or for 1 h after exposure to 0.4% glucose. Extracellular pH was determined ratiometrically using digital image analysis. Extracellular pH dropped rapidly in most examined sites after addition of glucose. Distinct pH microenvironments were observed within single biofilms. The variation in pH was similar between sites within the same biofilm and sites from different individuals. pH drop patterns did not differ between biofilms exposed to sucrose-free and sucrose-rich environments. The present study is the first of its kind to apply the combination of pH ratiometry and digital image analysis to systematically recor...Continue Reading

Citations

Apr 24, 2018·FEMS Yeast Research·Sebastian SchlaferJavier E Garcia
Sep 7, 2019·Journal of Oral Microbiology·Nizam AbdullahLakshman Perera Samaranayake
Jan 22, 2020·Small·Céline BurelRémi Dreyfus
May 26, 2020·Journal of Nanobiotechnology·Ella Yeor-DavidiFernando Patolsky
Feb 23, 2020·Journal of Microbiological Methods·Mathilde Frost KristensenSebastian Schlafer
Jun 19, 2021·NPJ Biofilms and Microbiomes·Birte HollmannKim R Hardie
Aug 23, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Sebastian SchlaferGerd Göstemeyer
Sep 28, 2021·ACS Applied Materials & Interfaces·Guang-Rong TanYong Zhang

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