Monitoring of hormonal drug effect in a single breast cancer cell using an estrogen responsive GFP reporter vector delivered by a nanoneedle

Biosensors & Bioelectronics
Sung-Woong HanJun Miyake

Abstract

In this study, we have evaluated a sensor system for a hormonal drug effect in a single cell level using a novel low invasive single cell DNA delivery technology using a nanoneedle. An estrogen responsive GFP reporter vector (pEREGFP9) was constructed and its estrogenic response activity was confirmed in breast cancer cells (MCF-7) using lipofection as the means of transferring the vector to the cells. The pEREGFP9 vector was delivered to a single MCF-7 using a nanoneedle and the effect of ICI 182,780, which is an antagonist of estrogen, was observed using the GFP expression level. By ICI 182,780 treatment, the fluorescence intensity of the GFP was decreased by 30-50% within 24h. This technology is the very first trial of single cell diagnosis and we are looking forward to applying it to precious single cell diagnosis in medical fields.

References

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Citations

Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
May 12, 2012·Advanced Drug Delivery Reviews·Yeu-Chun KimMark R Prausnitz
Jun 29, 2010·Biochimica Et Biophysica Acta·Kyungsuk YumYang K Xiang
Sep 11, 2014·ACS Applied Materials & Interfaces·Tae-Hyung KimJeong-Woo Choi
Dec 3, 2009·Journal of Molecular Recognition : JMR·Sung-Woong HanJun Miyake
Feb 22, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guojun Ma, Chengwei Wu

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