Monitoring of Legionella pneumophila viability after chlorine dioxide treatment using flow cytometry

Research in Microbiology
Pascale MustaphaSerge Riffard

Abstract

The viability of three Legionella pneumophila strains was monitored after chlorine dioxide (ClO2) treatment using a flow cytometric assay. Suspensions of L. pneumophila cells were submitted to increasing concentrations of ClO2. Culturable cells were still detected when using 4 mg/L, but could no longer be detected after exposure to 6 mg/L of ClO2, although viable but not culturable (VBNC) cells were found after exposure to 4-5 mg/L of ClO2. When testing whether these VBNC were infective, two of the strains were resuscitated after co-culture with Acanthamoeba polyphaga, but neither of them could infect macrophage-like cells.

References

Oct 1, 1995·Journal of Industrial Microbiology·J T WalkerC W Keevil
Jun 19, 2002·FEMS Microbiology Reviews·Michael SteinertJörg Hacker
Jul 5, 2002·Clinical Microbiology Reviews·Barry S FieldsRichard E Besser
Aug 28, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Arjun SrinivasanTrish M Perl
Jul 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Zhe ZhangVictor L Yu
Oct 14, 2008·Applied and Environmental Microbiology·Séverine AllegraSerge Riffard
Apr 5, 2011·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Yusen E LinVictor L Yu
Apr 28, 2012·Microbiology and Immunology·Mitsutoshi SenohYoshifumi Takeda
Jun 12, 2014·Frontiers in Microbiology·Laam LiSebastien P Faucher

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