Monitoring of pH changes in a live rat brain with MoS2 /PAN functionalized microneedles

The Analyst
Jin-Xiu ZhouGuo-Jun Zhang

Abstract

Monitoring the dynamic pH changes in vivo remains very essential to comprehend the function of pH in various physiological processes. In this study, we report a high-performance electrochemical pH microneedle based on an acupuncture needle (AN) for real-time monitoring of pH changes in a rat brain. The pH microneedle was prepared by a layer-to-layer assembly of molybdenum disulfide (MoS2) nanosheets and polyaniline (PAN), with an attempt to achieve a highly sensitive detection of hydrogen ions (H+). The as-prepared PAN/MoS2/AN exhibited a high Nernstian response of -51.2 mV per pH over a wide pH range from 3.0 to 9.0, and excellent selectivity toward pH against other potential interfering species in the brain. Moreover, the corresponding open circuit potential rapidly increased and decreased when Na2CO3 or NaH2PO4 was injected into the rat brain, respectively, demonstrating that the PAN/MoS2/AN has an excellent response toward pH changes in vivo. This work provides a new potentiometric method for real-time monitoring of dynamic pH changes in vivo with high reliability and stability.

References

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Citations

Aug 11, 2020·Analytical and Bioanalytical Chemistry·Raluca-Elena MunteanuSzilveszter Gáspár
Feb 11, 2021·ACS Sensors·Juan José García-GuzmánGastón A Crespo
Sep 4, 2020·Expert Opinion on Drug Delivery·Lindsay BarnumAli Tamayol
Jan 19, 2022·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Bilal DemirSabine Szunerits

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Methods Mentioned

BETA
biosensors
microsensor

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