Monitoring of Plant Protein Post-translational Modifications Using Targeted Proteomics

Frontiers in Plant Science
Borjana ArsovaBjörn Usadel

Abstract

Protein post-translational modifications (PTMs) are among the fastest and earliest of plant responses to changes in the environment, making the mechanisms and dynamics of PTMs an important area of plant science. One of the most studied PTMs is protein phosphorylation. This review summarizes the use of targeted proteomics for the elucidation of the biological functioning of plant PTMs, and focuses primarily on phosphorylation. Since phosphorylated peptides have a low abundance, usually complex enrichment protocols are required for their research. Initial identification is usually performed with discovery phosphoproteomics, using high sensitivity mass spectrometers, where as many phosphopeptides are measured as possible. Once a PTM site is identified, biological characterization can be addressed with targeted proteomics. In targeted proteomics, Selected/Multiple Reaction Monitoring (S/MRM) is traditionally coupled to simple, standard protein digestion protocols, often omitting the enrichment step, and relying on triple-quadruple mass spectrometer. The use of synthetic peptides as internal standards allows accurate identification, avoiding cross-reactivity typical for some antibody based approaches. Importantly, internal standards...Continue Reading

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Citations

Dec 21, 2019·FEMS Yeast Research·Maxime den RidderMartin Pabst
Dec 14, 2019·Plant Methods·Yahui LiuLanping Guo
Mar 23, 2019·International Journal of Molecular Sciences·Dong Qian, Yun Xiang
Feb 24, 2021·Emerging Topics in Life Sciences·Amanda L Smythers, Leslie M Hicks
Feb 19, 2021·Frontiers in Plant Science·Petr DvořákTomáš Takáč
Jul 23, 2021·Molecular Omics·Sara HamzelouPaul A Haynes
Jul 21, 2021·Journal of Experimental Botany·Myrthe PraatMartijn van Zanten

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Methods Mentioned

BETA
environmental stresses

Software Mentioned

Skyline
Musite
PhosPhAt
P3DB

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