Monitoring of RBC rheology after cryopreservation to detect autologous blood doping in vivo? A pilot study

Clinical Hemorheology and Microcirculation
Daniel A BizjakMarijke Grau

Abstract

Autologous blood doping (ABD) is applied to improve performance capacity. ABD includes blood donation, red blood cell (RBC) storage at -80°C and re-infusion prior to or during competition. ABD is not directly detectable with current detection techniques. Since cryopreservation is known to affect RBC physiology in vitro, the aim of the study was to examine whether these alterations are detectable in vivo. Blood from six healthy male donors was transferred into conventional blood bags, cryopreserved, stored for 18 weeks at -80°C and re-infused with a RBC volume corresponding to ∼4% of total blood volume into respective donor. RBC physiology parameters were measured before blood donation/re-infusion, and 0/1/2/6/24/48/72 h and 1 w post re-infusion. RBC parameters and age markers were unaffected during intervention. RBC deformability increased from pre-blood-sampling to pre-re-infusion while deformability and viscosity values remained unaltered post re-infusion. RBC nitric oxide associated analytes, metabolic parameters and electrolyte concentrations remained unaffected. The data of this pilot study indicate that the increase in RBC deformability might be related to neoformation of RBC after blood donation. The lack of changes in t...Continue Reading

References

Jun 1, 1992·Annals of Hematology·A LorentzJ R Duchow
Aug 1, 1972·Journal of Applied Physiology·B EkblomB Gullbring
Nov 1, 1983·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·H MairbäurlH Pessenhofer
May 13, 1999·Biochimica Et Biophysica Acta·M Kelm
Jan 31, 2004·British Journal of Sports Medicine·S Leigh-Smith
Dec 22, 2005·Blood·Petra KleinbongardMalte Kelm
Jul 26, 2006·Free Radical Biology & Medicine·Mildred M PelletierAndré Dejam
Jul 29, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·C Robert Valeri, Gina Ragno
Jul 27, 2007·Best Practice & Research. Clinical Anaesthesiology·Emre Almac, Can Ince
Jun 9, 2009·Experimental Hematology·Walter Fried
Jun 22, 2010·Transfusion·Sandra HenkelmanWillem Van Oeveren
Mar 26, 2011·Journal of Cellular and Molecular Medicine·Yao-Xiong HuangMan Luo
Oct 9, 2012·Clinical Hemorheology and Microcirculation·Philippe ConnesOguz K Baskurt
Oct 31, 2012·Clinical Hemorheology and Microcirculation·Oguz K Baskurt, Herbert J Meiselman
Nov 2, 2012·Blood Transfusion = Trasfusione Del Sangue·Giovanni LombardiFabian Sanchis-Gomar
Nov 2, 2012·American Journal of Hematology·Robert S FrancoRobert M Cohen
Mar 2, 2013·Anesthesia and Analgesia·Steven M FrankViachaslau M Barodka
Aug 13, 2013·Analytical and Bioanalytical Chemistry·Torben Pottgiesser, Yorck Olaf Schumacher
Jun 15, 2014·Clinical Hemorheology and Microcirculation·Marijke GrauWilhelm Bloch
Jul 1, 2014·The Journal of Trauma and Acute Care Surgery·David A HamptonMartin A Schreiber
Sep 20, 2016·Blood Transfusion = Trasfusione Del Sangue·Alex ChangTimothy Pritts
Mar 25, 2018·Internal and Emergency Medicine·Nicola LambertiFabio Manfredini

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Citations

Nov 14, 2020·Drug Testing and Analysis·Mario ThevisHans Geyer

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