Monitoring of recombinant survival motor neuron protein using fiber-optic surface plasmon resonance

The Analyst
Jean-Francois MassonKarl S Booksh

Abstract

Spinal muscular atrophy (SMA) is the leading genetic cause of infant mortality. SMA is caused by the homozygous loss of the survival motor neuron 1 (SMN1) gene. A nearly identical copy gene exists known as SMN2, however, due to an aberrant splicing event, the SMN2 gene fails to produce sufficient full-length protein to protect against disease development in the absence of SMN1. While a number of compounds have recently been identified that can stimulate full-length survival motor neuron (SMN) expression from the nearly identical copy SMN2, one of the difficulties has been the lack of a highly reproducible and quantitative means to measure the levels of SMN protein. To develop a technique that allows the rapid and highly sensitive measurement of SMN protein, a Surface Plasmon Resonance (SPR) application has been developed. The ability to quantify unassociated SMN protein and monitor the binding of SMN with other proteins in solution using a SPR sensor in less than 15 min and at low ng mL(-1) levels in HEPES Buffer Saline (HBS) has been achieved. The detection limit for the specific binding of SMN in HBS pH 7.4 solution is 0.99 ng mL(-1) with non-specific binding accounting for approximately 30% of the signal. Quantification of S...Continue Reading

Citations

Aug 26, 2014·Assay and Drug Development Technologies·Jonathan J CherryJill Jarecki
Nov 7, 2006·Analytical and Bioanalytical Chemistry·Jean-Francois MassonKarl S Booksh
Dec 16, 2006·Analytical and Bioanalytical Chemistry·Denis HabauzitBenoit Roig
May 25, 2005·The Analyst·Yoon-Chang KimKarl S Booksh
Aug 11, 2015·Scientific Reports·Yun LiuWei Peng
Nov 30, 2006·Applied Spectroscopy·Jean-Francois MassonKarl S Booksh
Oct 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Olivier R Bolduc, Jean-François Masson
Oct 20, 2007·Analytical Chemistry·Jean-Francois MassonBoris Mizaikoff

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