Monitoring of renal venous PO2 and kidney oxygen consumption in rats by a near-infrared phosphorescence lifetime technique

American Journal of Physiology. Renal Physiology
Egbert G MikCan Ince

Abstract

Renal oxygen consumption (Vo(2,ren)) is an important parameter that has been shown to be influenced by various pathophysiological circumstances. Vo(2,ren) has to be repeatedly measured during an experiment to gain insight in the dynamics of (dys)regulation of oxygen metabolism. In small animals, the classical approach of blood gas analysis of arterial and venous blood samples is only limitedly applicable due to fragile vessels and a low circulating blood volume. We present a phosphorescence lifetime technique that allows near-continuous measurement of renal venous Po(2) (vPo(2)) and Vo(2,ren) in rats. The technique does not rely on penetration of the blood vessel, but uses a small reflection probe. This probe is placed in close proximity to the renal vein for detection of the oxygen-dependent phosphorescence of the injected water-soluble near-infrared phosphor Oxyphor G2. The technique was calibrated in vitro and the calibration constants were validated in vivo in anesthetized and mechanically ventilated male Wistar rats. The hemoglobin saturation curve and its pH dependency were determined for calculation of renal venous oxygen content. The phosphorescence technique was in good agreement with blood gas analysis of renal venous...Continue Reading

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Citations

Jul 31, 2008·The Journal of Physical Chemistry. a·Artem Y LebedevSergei A Vinogradov
Mar 17, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Paola CeroniSergei A Vinogradov
Nov 6, 2008·Clinical and Experimental Pharmacology & Physiology·Roger G EvansPaul M O'Connor
May 6, 2009·Clinical and Experimental Pharmacology & Physiology·Tanja JohannesCan Ince
May 29, 2009·Clinical and Experimental Pharmacology & Physiology·Roger G Evans, Clive N May
Jan 1, 2010·Sensors·Samantha M GristKaren C Cheung
Aug 13, 2015·Intensive Care Medicine Experimental·Emre AlmacCan Ince
May 23, 2014·Analytical Chemistry·Emmanuel RoussakisSergei A Vinogradov
Dec 9, 2015·Scientific Reports·Yosuke HirakawaMasaomi Nangaku
Sep 26, 2008·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Xiang HeDmitriy A Yablonskiy
Nov 25, 2011·Journal of Biophotonics·Sander I A BodmerEgbert G Mik
Oct 15, 2010·Biotechnology Progress·Angela Guaccio, Paolo A Netti
Mar 1, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Zhongwei ZhangRalph P Mason
Aug 2, 2015·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Lara Zafrani, Can Ince
Nov 13, 2014·Journal of Clinical Monitoring and Computing·Floor A HarmsEgbert G Mik
Jan 7, 2010·Optics Express·Mohammad A YaseenDavid A Boas
Mar 22, 2015·Seminars in Nephrology·Lara ZafraniCan Ince
May 23, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Bulent ErginCan Ince
Dec 10, 2016·Journal of Applied Physiology·Philippe GuerciCan Ince
Mar 9, 2017·Frontiers in Physiology·Yosuke HirakawaMasaomi Nangaku
Oct 26, 2018·Physiological Reviews·Thomas P Keeley, Giovanni E Mann
Mar 7, 2014·American Journal of Physiology. Renal Physiology·Amany AbdelkaderRoger G Evans
Dec 21, 2016·Critical Care : the Official Journal of the Critical Care Forum·Lara ZafraniCan Ince
Jan 21, 2021·BMJ Case Reports·Floor A HarmsEgbert G Mik

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