Monitoring of the deuterated and nondeuterated forms of levodopa and five metabolites in plasma and urine by LC-MS/MS

Bioanalysis
Nico C van de MerbelRay Bakhtiar

Abstract

To compare pharmacokinetics, metabolism and excretion of levodopa and a triply deuterated form, which is being developed as an improved treatment for Parkinson's disease, methods were needed for quantification of the deuterated and nondeuterated forms of levodopa and five metabolites in human plasma and urine. Results: The natural heavy isotopes in the nondeuterated compounds caused an absolute contribution of up to 100% in the response of the deuterated compounds. Similarly, heavy isotopes in the deuterated analytes contributed to the response of the internal standards, but this did not affect the reliability of the results. Conclusion: Deuterated and nondeuterated analytes can be quantified together by LC-MS/MS, but overestimation of the concentrations of the deuterated molecules may be unavoidable and a careful interpretation of the concentration data is essential.

References

Jun 20, 1998·Neurology·W C Koller, M G Rueda
Apr 12, 2005·Movement Disorders : Official Journal of the Movement Disorder Society·Joseph Jankovic
Jun 20, 2008·Experimental Neurology·Torun MalmlöfBjörn Schilström
Jun 18, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aimin TanAnn Lévesque
Apr 4, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Venkatraman Junnotula, Hermes Licea-Perez
Nov 29, 2013·Journal of Labelled Compounds & Radiopharmaceuticals·F Peter Guengerich
Dec 4, 2013·Journal of Medicinal Chemistry·Thomas G Gant
Jul 30, 2014·Expert Opinion on Therapeutic Patents·Graham S Timmins
May 30, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xian-En ZhaoShuying Liu
Sep 16, 2015·Journal of Pharmaceutical and Biomedical Analysis·Daping ZhangDanielle R Rios
Sep 16, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nico C van de MerbelRay Bakhtiar

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