Monitoring of veterinary drug residues by a combination of continuous flow techniques and column-switching high-performance liquid chromatography. I. Sulphonamides in egg, meat and milk using post-column derivatization with dimethylaminobenzaldehyde

Journal of Chromatography
M M AertsU A Brinkman

Abstract

By using a combination of a low-pressure continuous flow module and a column-switching high-performance liquid chromatographic system, procedures have been developed for the automated residue analysis of sulphonamides and selected other drugs in meat, egg and milk. Aqueous extracts are purified by on-line dialysis and subsequent trace enrichment on a short column containing silica-based or polymer material. After backflush to the analytical column, detection is performed either at 280 nm or, after post-column derivatization with p-dimethylaminobenzaldehyde, at 450 nm. With these procedures the fully automated determination of both polar and apolar sulphonamides and dapsone above 5-20 micrograms/kg is possible. Coefficients of variation are 4-10%, recoveries compared to standards are 85-90%. The methods developed were tested in routine veterinary drug control.

Citations

Aug 1, 1994·Talanta·L F Capitan-VallveyJ J Berzas-Nevado
Sep 22, 1993·Journal of Chromatography·P Campíns-FalcóA Sevillano-Cabeza
May 5, 1995·Journal of Chromatography. B, Biomedical Applications·M M AertsU A Brinkman
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·M J MengelersA S van Miert
Jan 1, 1991·Journal of Pharmaceutical and Biomedical Analysis·J J Berzas NevadoL F Capitan-Vallvey
Jul 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·J J Berzas NevadoG Castañeda Peñalvo
Feb 1, 1992·Journal of the American Society for Mass Spectrometry·J R PerkinsK B Tomer
Feb 17, 1998·Journal of Chromatography. a·C E LinS W Wang
Oct 20, 1999·Journal of Chromatography. a·N C Van de Merbel
Aug 2, 2002·Journal of Pharmaceutical and Biomedical Analysis·Leticia López-MartínezMa Lourdes Yepez-Murrieta
Jun 8, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David N HellerMichael H Thomas
Jul 1, 1993·Food Additives and Contaminants·B Roudaut, M Garnier
Jul 16, 2010·Analytical and Bioanalytical Chemistry·Ruixia GaoYukui Zhang

Related Concepts

p-dimethylaminobenzaldehyde
Metazoa
Benzaldehydes
Bos indicus
High Pressure Liquid Chromatography Procedure
Drug Residues
Egg Food Product
Indicators
Meat
Spectrophotometry, Ultraviolet

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