Monitoring phases and phase transitions in phosphatidylethanolamine monolayers using active interfacial microrheology

Soft Matter
Saba GhazviniPrajnaparamita Dhar

Abstract

Active interfacial microrheology is a sensitive tool to detect phase transitions and headgroup order in phospholipid monolayers. The re-orientation of a magnetic nickel nanorod is used to explore changes in the surface rheology of 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE) and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), which differ by two CH2 groups in their alkyl chains. Phosphatidylethanolamines such as DLPE and DMPE are a major component of cell membranes in bacteria and in the nervous system. At room temperature, DLPE has a liquid expanded (LE) phase for surface pressure, Π < ∼38 mN m(-1); DMPE has an LE phase for Π < ∼7 mN m(-1). In their respective LE phases, DLPE and DMPE show no measurable change in surface viscosity with Π, consistent with a surface viscosity <10(-9) N s m(-1), the resolution of our technique. However, there is a measurable, discontinuous change in the surface viscosity at the LE to liquid condensed (LC) transition for both DLPE and DMPE. This discontinuous change is correlated with a significant increase in the surface compressibility modulus (or isothermal two-dimensional bulk modulus). In the LC phase of DMPE there is an exponential increase in surface viscosity with Π consi...Continue Reading

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Citations

Jul 22, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Christian SchwiegerAlfred Blume
Jan 27, 2017·Soft Matter·A K SachanJ A Zasadzinski
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Zsuzsa AkosAndras Czirok
Oct 12, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Saba GhazviniPrajnaparamita Dhar
Mar 24, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·M OrczykG Brezesinski

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