Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses

Frontiers in Immunology
Anja Ten BrinkeSilvia Gregori

Abstract

Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation. One of the critical issues to elucidate the mechanisms responsible for success or failure of these therapies and define the specificity of the therapy is the evaluation of the Ag-specific T-cell responses. Several efforts have been made to develop suitable and reproducible assays. Here, we focus on dye-based proliferation assays. We highlight with practical examples the fundamental issues to take into consideration for implementation of an effective and sensitive dye-based proliferation assay to monitor Ag-specific responses in patients. The most critical points were used to design a road map to set up and analyze the optimal assay to assess Ag-specific T-cell responses in patients undergoing different treatments. This is the first step to optimize monitoring of tolerance induction, allowing comparison of outcomes of different clinical studies. The road map can also be applied to other therapeutic interventions, not limited to tolerance inducti...Continue Reading

References

Oct 4, 1996·Science·J D AltmanM M Davis
Nov 22, 2002·Journal of the American Society of Nephrology : JASN·Wan Fai NgRobert I Lechler
Feb 14, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·William W Kwok
Dec 9, 2003·Journal of Immunological Methods·Stuart I ManneringLeonard C Harrison
May 9, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Junbao YangWilliam W Kwok
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ivana Durinovic-BellóGrete Sønderstrup
Jan 24, 2007·Immunology·Marina ZafranskayaMatthias Eberl
Jan 25, 2008·The New England Journal of Medicine·Tatsuo KawaiDavid H Sachs
Sep 5, 2008·Current Protocols in Cytometry·Jacek M Witkowski
Sep 13, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul K WallaceKatharine A Muirhead
Feb 25, 2009·Current Protocols in Immunology·Christopher R ParishHilary S Warren
May 27, 2010·The Journal of Clinical Investigation·Pervinder SagooRobert I Lechler
Sep 18, 2010·Clinical and Experimental Immunology·S I ManneringUNKNOWN Immunology of Diabetes Society T-Cell Workshop Committee
Jan 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mario Roederer
Mar 1, 2012·Journal of Immunological Methods·Benjamin J C Quah, Christopher R Parish
Nov 6, 2012·Cold Spring Harbor Perspectives in Biology·Jeffrey A Bluestone, Hélène Bour-Jordan
Jan 16, 2013·Journal of Immunological Methods·Kimberly A LivingstonCharles B Stephensen
Jun 4, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Anastasia G DaifotisKevan C Herold
Feb 20, 2014·Frontiers in Immunology·Rosa BacchettaMaria-Grazia Roncarolo
Jun 10, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Per MaritsSarah Thunberg
Jul 6, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Norma Segovia-GamboaCarmen Sánchez-Torres
Apr 22, 2015·JAMA : the Journal of the American Medical Association·Ezio BonifacioUNKNOWN Pre-POINT Study Group
Jul 3, 2015·Journal of Multiple Sclerosis·Sushmita SinhaNitin J Karandikar
Sep 12, 2015·Science Translational Medicine·Piotr TrzonkowskiBirgit Sawitzki
Jan 14, 2016·Transplantation·Susan DeWolfMegan Sykes
Jan 29, 2016·Mediators of Inflammation·Anja Ten BrinkeEva M Martinez-Caceres

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Citations

Oct 17, 2019·Clinical and Experimental Immunology·N M ShahM R Johnson
Mar 12, 2019·Frontiers in Immunology·Anja Ten BrinkeEva Martinez-Caceres
Aug 28, 2020·Journal for Immunotherapy of Cancer·Tal KanYuval Shaked
Nov 15, 2019·Critical Care : the Official Journal of the Critical Care Forum·McKenzie K HollenPhilip A Efron
Jan 12, 2021·Frontiers in Immunology·Yassmin MusthaffaRanjeny Thomas
Oct 15, 2020·Journal of Clinical Immunology·Tannaz Moeini ShadReza Yazdani
Apr 20, 2021·Frontiers in Immunology·Dorota Iwaszkiewicz-GrzesPiotr Trzonkowski
Sep 16, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Satoshi OkamoriKoichi Fukunaga

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
flow cytometry
FCS
FACS
Assay
PMA
ELISA

Software Mentioned

FACS Diva
FCS Express
FASCIA
FlowJo

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