Monitoring the cellular effects of HMG-CoA reductase inhibitors in vitro and ex vivo

Arteriosclerosis, Thrombosis, and Vascular Biology
Iwona CichaMargarete Goppelt-Struebe

Abstract

Inhibition of 3hydroxy3methylglutaryl-coenzyme A (HMG-CoA) reductase by statins and the subsequent reduction in Rho protein isoprenylation inactivates these important signaling molecules. The purpose of this study was to directly monitor statin effects on Rho proteins. We used biphasic Triton X-114 system, 1-dimensional isoelectric focusing, and 2D-electrophoresis for the separation of modified and nonmodified Rho proteins. These methods were evaluated in human fibroblasts treated with simvastatin. 2D-electrophoresis, which proved to be the most sensitive method, revealed 2 major spots of identical molecular weight but different isoelectric points, with the more basic spot representing the carboxymethylated form of RhoA. In control cells, 90% of RhoA was fully modified (carboxymethylated). After treatment with simvastatin, a significant shift toward the unmethylated form was observed, representing inhibition of isoprenylation, which is a prerequisite to further modification. Similar shifts were observed for Rac1 and Cdc42. In freshly isolated peripheral blood mononuclear cells, a shift toward nonmodified RhoA was observed after treatment with atorvastatin in vitro and in vivo. There was a significant increase in unmethylated Rh...Continue Reading

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Citations

Mar 3, 2012·Cardiovascular Drugs and Therapy·Xiaoman LiuFengshuang An
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Manish RauthanMarc Pilon
Jul 11, 2006·Journal of Medicinal Food·M D RodríguezA Gómez
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Jul 24, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hedwich F Kuipers, Peter J van den Elsen
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