Monitoring the effect of mild ischemia with a built-in light-emitting diode contact lens electrode and a low-cost custom-made apparatus

Physiological Measurement
A FleischmanO Geyer

Abstract

Electroretinography (ERG) is widely used in clinical work and research to assess the retinal function. We evaluated an easy to build ERG setup adapted for small animals comprising two contact lens electrodes with a built-in light-emitting diode and a custom-made amplification system. The system's sensitivity was tested by monitoring ERG in albino rat eyes subjected to mild ischemia. Flash ERG was recorded by two contact lens electrodes positioned on the rat's corneas and used alternately as test or reference. The a- and b-wave amplitudes, a-wave latency, b-wave implicit time and oscillatory potentials (OPs) were analyzed. Ischemia was achieved by elevating the intraocular pressure in the eye's anterior chamber. ERG was recorded on post-ischemia (PI) days -1, 1, 3 and 7. Morphological changes were analyzed on hematoxylin/eosin stained 5 µm sections of control 7d PI retinas. In control eyes, ERG exhibited a pattern similar to a standard recording. Retinas subjected to mild ischemia preserved ordered layered morphology, exhibiting approximately 30% loss of ganglion cells and no changes in gross morphology. By day 3 PI, ischemia caused an increase in the a-wave amplitude (from 34.9 ± 2.7 to 45.4 ± 4.3 µV), a decrease in the b-wave ...Continue Reading

References

Jan 1, 1991·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·E R BüchiJ Fu
Apr 1, 1997·Experimental Eye Research·F Block, M Schwarz
Oct 12, 2001·Acta Ophthalmologica Scandinavica·S MizunoyaT Hirose
Jan 15, 2003·Current Eye Research·Bing LiWilliam Holt
Jan 31, 2003·Investigative Ophthalmology & Visual Science·Fei WangSheldon S Miller
Mar 26, 2003·Brain Research·Shinji YonedaHideaki Hara
May 10, 2003·Documenta Ophthalmologica. Advances in Ophthalmology·Kazuki KuniyoshiYoshikazu Shimomura
May 27, 2003·Investigative Ophthalmology & Visual Science·Sinisa D GrozdanicIoana M Sonea
Feb 10, 2004·Progress in Retinal and Eye Research·Neville N OsborneJosé Melena
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Heather A Hancock, Timothy W Kraft
Apr 13, 2004·Retina·Mauricio MaiaMark S Humayun
Apr 24, 2004·Documenta Ophthalmologica. Advances in Ophthalmology·Mieko TsuruokaMasanori Hayashi
Oct 27, 2004·Investigative Ophthalmology & Visual Science·Shigeki MachidaYutaka Tazawa
Dec 30, 2004·Investigative Ophthalmology & Visual Science·Bang V BuiBrad Fortune
Jun 9, 2005·The Journal of Veterinary Medical Science·Seiya MaeharaTadao Kotani
Sep 6, 2008·The Journal of Veterinary Medical Science·Hyun-Ah KimKang-Moon Seo

❮ Previous
Next ❯

Citations

Jun 18, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Anat FleischmanOrna Geyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.