Monitoring the fibrolytic potential of microbial ecosystems from domestic and wild ruminants browsing tanniferous forages

Animal Nutrition
Nokwethemba Nqobile Philile Msimango, Fabian Nde Fon

Abstract

Although the rumen microbiome has been reported to synthesize a rich source of symbiotic enzymes (exocellulase, endocellulase, hemicellulase and cellobiase), the digestion of tropical C4 grasses and browses by ruminants is still limited. Therefore, this study aimed to unveil potential fibrolytic microbial ecosystems from giraffe, kudu, impala and consortia (A1 [giraffe + kudu], A2 [giraffe + impala], A3 [kudu + impala], and A4 [giraffe + kudu + impala]) browsing tanniferous plants, which can be used to improve forage utilization in domesticated goat. Crude protein enzyme extracts (CPZ) from fresh faecal samples were precipitated by 60% ammonium sulphate and assayed for exocellulase, endocellulase and hemicellulase by incubating with crystalline cellulose, carboxymethyl cellulose and xylan at 38 °C with optimum pH of 5.5 to 6.5 for 1, 2, and 48 h, respectively. Enzyme specific activities were defined as μg of reducing sugar/mg CPZ. In vitro fermentation study was done by transferring 33 mL of fresh faecal inoculum into 67 mL of salivary buffer containing 1 g Acacia sieberiana and incubating for 72 h at 38 °C. Apparent degradability (APDeg), true degradability (TD), neutral detergent fibre degradability (NDFdeg), acid detergent f...Continue Reading

References

Aug 16, 2002·International Microbiology : the Official Journal of the Spanish Society for Microbiology·J PérezJ Martínez
Feb 1, 1988·Applied and Environmental Microbiology·J B OwolabiR A Warren
Apr 2, 2008·Annals of the New York Academy of Sciences·David B Wilson
Sep 28, 2010·Critical Reviews in Biotechnology·Mehdi DashtbanWensheng Qin
May 3, 2011·Current Opinion in Microbiology·David B Wilson
Jul 4, 2012·Biotechnology for Biofuels·Svein Jarle HornVincent Gh Eijsink
Jan 6, 2015·Asian-Australasian Journal of Animal Sciences·B S Gemeda, A Hassen

❮ Previous
Next ❯

Software Mentioned

Statistically Analysis System ( SAS

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here