Monitoring the kinetics of Bacillus subtilis endospore germination via surface-enhanced Raman scattering spectroscopy

Analytical Chemistry
Jacquitta K DanielsGeorge Chumanov

Abstract

Mirror sandwich SERS substrates (M3S) were used to monitor the kinetics of Bacillus subtilis endospore germination. The sandwich configuration of the substrates allows real-time observation of germination in samples that contained only several hundred endospores. The enhancement provided by the substrates is attributed to the enhanced local electromagnetic field that originates from coupling between the Ag nanoparticles and the underlying metal film as well as from coupling between the Ag nanoparticles themselves. The germination kinetics at varying concentrations of l-alanine and different temperatures were studied by monitoring the intensity and growth of the Raman peak at 1010 cm(-1), which is characteristic of dipicolinic acid. A total of four concentrations (50, 75, 100, and 150 mM) of l-alanine and three different temperatures (30, 37, and 55 degrees C) were investigated.

References

Dec 21, 1977·Biochemical and Biophysical Research Communications·D P Rossignol, J C Vary
Dec 16, 1998·Journal of Bacteriology·M O Clements, A Moir
Aug 1, 2002·Journal of Clinical Microbiology·Constance A BellFranklin R Cockerill
Sep 14, 2002·Infection and Immunity·John A W Ireland, Philip C Hanna
Sep 10, 2005·Applied and Environmental Microbiology·Sandra C StringerMichael W Peck
Jul 21, 2006·The Journal of Physical Chemistry. B·Jacquitta K Daniels, George Chumanov

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Citations

Apr 4, 2009·Analytical and Bioanalytical Chemistry·Stephen D Hudson, George Chumanov
Oct 27, 2007·Analytical and Bioanalytical Chemistry·Joke De GelderPaul De Vos
Feb 15, 2011·Food Microbiology·S C StringerM W Peck
Mar 5, 2016·Annual Review of Food Science and Technology·Marjon H J Wells-BennikTjakko Abee
Nov 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshinori MorisakuHiroharu Yui
Jul 9, 2011·Journal of Separation Science·Petra Juskova, Frantisek Foret
Nov 16, 2017·Biosensors·Pamela A Mosier-Boss
Aug 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·John C HeckelGeorge Chumanov
Sep 28, 2006·Journal of the American Chemical Society·David D EvanoffGeorge Chumanov

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