Monitoring the mechanism of anti-cancer agents to inhibit colorectal cancer cell proliferation: Enzymatic biosensing of glucose combined with molecular docking.

Enzyme and Microbial Technology
Samira YazdanparastSeyyed Kazem Sabbagh

Abstract

Glucose, a major energy source in cellular metabolism, has a significant role in cell growth. The increase in glucose uptake is a distinguishing hallmark in cancer cells. A key step in glucose utilization is the transport of glucose to the cancer cells for supplying their additional energy. The glucose transporter (or GLUT) family is a membrane protein which facilitates the uptake of glucose in most cancer cell types. Given the increased glucose level in cancer cells and the regulatory role of GLUTs in glucose uptake, it is required to combine both experimental and theoretical studies to develop new methods to monitor cell proliferation. Herein, for the first time, a new strategy was proposed to evaluate the cell proliferation of HT-29 based on glucose consumption in the presence of resveratrol (RSV) as an anticancer agent. A hybrid nanocomposite of carbon nanofibers and nitrogen-doped graphene quantum dots was used to design an enzymatic sensor for the selective and sensitive determination of glucose in cancer cells. The results obtained from the voltammetric technique were compared with the conventional colorimetric assay. A good correlation was observed between the proliferation rate and glucose utilization by cancer cells. ...Continue Reading

References

Mar 10, 2001·Apoptosis : an International Journal on Programmed Cell Death·K H Moley, M M Mueckler
Aug 30, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Fiona M YoungBarbara J S Sanderson
Nov 10, 2007·Chemical Research in Toxicology·Timo LaaksonenJouni Hirvonen
Jan 25, 2011·Nature Reviews. Cancer·Rob A CairnsTak W Mak
Mar 2, 2011·Nature Reviews. Drug Discovery·Oliver KeppGuido Kroemer
Jul 17, 2012·Biosensors & Bioelectronics·Binesh UnnikrishnanShen-Ming Chen
Jul 18, 2012·Chemical Research in Toxicology·Amara L HolderCatherine P Koshland
Jan 2, 2014·Pharmacological Reviews·Gregory SliwoskiEdward W Lowe
Jul 6, 2014·Applied Biochemistry and Biotechnology·A Muthurasu, V Ganesh
Jul 6, 2014·Biosensors & Bioelectronics·Kuo-Yuan Hwa, Boopathi Subramani
Aug 13, 2014·Biosensors & Bioelectronics·Hua LiCai-Ling Xu
Dec 5, 2014·ACS Chemical Biology·Henrik Daub
Jul 29, 2015·Enzyme and Microbial Technology·Rajkumar DevasenathipathyBo-Jun Chen
Mar 15, 2016·Cancer Treatment Reviews·Francesco MassariRodolfo Montironi
Apr 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Khyati KapoorRobert M Stroud
Sep 23, 2016·Biosensors & Bioelectronics·Sasya MadhurantakamUma Maheswari Krishnan
Apr 1, 2018·Enzyme and Microbial Technology·A K M KafiMaxwell J Crossley
May 11, 2018·Analytical Chemistry·Bella H NeufeldMelissa M Reynolds
Mar 2, 2019·International Journal of Radiation Biology·Peter VaupelArnulf Mayer
Apr 24, 2019·Nanomaterials·Susana CampuzanoJosé M Pingarrón
Oct 18, 2019·Frontiers in Bioengineering and Biotechnology·Zanib ChaudharyAmirali Popat
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dominique Vervandier-Fasseur, Norbert Latruffe
Jul 23, 2020·Nature Metabolism·Ralph J DeBerardinis, Navdeep S Chandel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.