Monitoring the mitochondrial transmembrane potential with the JC-1 fluorochrome in programmed cell death during mesophyll leaf senescence

Protoplasma
E SimeonovaA Mostowska

Abstract

Analysis of the mitochondrial transmembrane potential (Delta Psi(m)) with the help of the JC-1 fluorochrome (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide) during mesophyll leaf senescence was performed in order to determine whether a reduction of Delta Psi(m) takes place during mesophyll senescence and whether plant mitochondria, like mammalian ones, might be involved in the induction of programmed cell death. Fluorescence analysis of mesophyll protoplasts of Pisum sativum in a confocal microscope, fluorescent spectra analysis and time dependence of fluorescence intensity of monomers and of J-aggregates revealed that JC-1 is incorporated and accumulated specifically in plant mitochondria. Analysis of Delta Psi(m) during mesophyll protoplast senescence revealed that two subpopulations of mitochondria which differ in Delta Psi(m) exist in all analyzed stages of leaf senescence. The first subpopulation contains mitochondria with red fluorescence of J-aggregates due to an unperturbed high Delta Psi(m). The second subpopulation comprises mitochondria with green fluorescence of monomers due to a low Delta Psi(m), proving total depolarization of mitochondrial membranes. Fluorescence analysis demonstrated t...Continue Reading

Citations

Nov 28, 2006·Biochemistry. Biokhimii︠a︡·E V DzyubinskayaV D Samuilov
Jun 24, 2011·PloS One·Rajesh ChandramohanadasSubra Suresh
Sep 23, 2014·Plant Physiology and Biochemistry : PPB·Petr BabulaIvo Provaznik
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Nan YaoJean T Greenberg
Dec 17, 2011·Plant, Cell & Environment·Bastiaan BrouwerPer Gardeström
Jun 30, 2004·Psychology and Aging·Teri A GarstkaMary Lee Hummert
Nov 7, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Haijie ChenQianling Zhang

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