Monitoring the multitask mechanism of DNase I activity using graphene nanoassemblies

Bioconjugate Chemistry
Neil RobertsonMehmet V Yigit

Abstract

Here we have demonstrated that graphene serves as a remarkable platform for monitoring the multitask activity of an enzyme with fluorescence spectroscopy. Our studies showed that four different simultaneous enzymatic tasks of DNase I can be observed and measured in a high throughput fashion using graphene oxide and oligonucleotide nanoassemblies. We have used phosphorothioate modified oligonucleotides to pinpoint the individual and highly specific functions of DNase I with single stranded DNA, RNA, and DNA/DNA and DNA/RNA duplexes. DNase I resulted in fluorescence recovery in the nanoassemblies and enhanced the intensity tremendously in the presence of sequence specific DNA or RNA molecules with different degrees of amplification. Our study enabled us to discover the sources of this remarkable signal enhancement, which has been used for biomedical applications of graphene for sensitive detection of specific oncogenes. The significant difference in the signal amplification observed for the detection of DNA and RNA molecules is a result of the positive and/or reductive signal generating events with the enzyme. In the presence of DNA there are four possible ways that the fluorescence reading is influenced, with two of them resulti...Continue Reading

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Citations

Aug 26, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Neil M RobertsonMehmet V Yigit
May 25, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Srivathsan V RanganathanAlan A Chen
May 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Nidhi NanduMehmet V Yigit
May 8, 2018·ACS Sensors·Erik E AugspurgerMehmet V Yigit

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