Monitoring Therapeutic Treatments against Burkholderia Infections Using Imaging Techniques

Pathogens
Tiffany M MottAlfredo G Torres

Abstract

Burkholderia mallei, the etiologic agent of glanders, are Category B select agents with biothreat potential, and yet effective therapeutic treatments are lacking. In this study, we showed that CpG administration increased survival, demonstrating protection in the murine glanders model. Bacterial recovery from infected lungs, liver and spleen was significantly reduced in CpG-treated animals as compared with non-treated mice. Reciprocally, lungs of CpG-treated infected animals were infiltrated with higher levels of neutrophils and inflammatory monocytes, as compared to control animals. Employing the B. mallei bioluminescent strain CSM001 and the Neutrophil-Specific Fluorescent Imaging Agent, bacterial dissemination and neutrophil trafficking were monitored in real-time using multimodal in vivo whole body imaging techniques. CpG-treatment increased recruitment of neutrophils to the lungs and reduced bioluminescent bacteria, correlating with decreased bacterial burden and increased protection against acute murine glanders. Our results indicate that protection of CpG-treated animals was associated with recruitment of neutrophils prior to infection and demonstrated, for the first time, simultaneous real time in vivo imaging of neutro...Continue Reading

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Citations

Oct 22, 2014·Journal of Immunology Research·Julia A TreeCaroline A Rowland
Mar 25, 2016·Expert Review of Vaccines·Sophie A AschenbroichRobert J Hogan
Dec 31, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xinlin YangQuanjun Cui
Oct 21, 2020·Emerging Infectious Diseases·Alexandria E KestersonHenry S Heine

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Methods Mentioned

BETA
imaging techniques
Fluorescence
flow cytometry
FCS
imaging technique
Flow

Software Mentioned

Sbrana
FCS
FCS Express
FACS DIVA
Graph Pad
GraphPad

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