Monitoring urban beaches with qPCR vs. culture measures of fecal indicator bacteria: Implications for public notification

Environmental Health : a Global Access Science Source
Samuel DorevitchIra Heimler

Abstract

The United States Environmental Protection Agency has established methods for testing beach water using the rapid quantitative polymerase chain reaction (qPCR) method, as well as "beach action values" so that the results of such testing can be used to make same-day beach management decisions. Despite its numerous advantages over culture-based monitoring approaches, qPCR monitoring has yet to become widely used in the US or elsewhere. Considering qPCR results obtained on a given day as the best available measure of that day's water quality, we evaluated the frequency of correct vs. incorrect beach management decisions that are driven by culture testing. Beaches in Chicago, USA, were monitored using E. coli culture and enterococci qPCR methods over 894 beach-days in the summers of 2015 and 2016. Agreement in beach management using the two methods, after taking into account agreement due to chance, was summarized using Cohen's kappa statistic. No meaningful agreement (beyond that expected by chance) was observed between beach management actions driven by the two pieces of information available to beach managers on a given day: enterococci qPCR results ofsamples collected that morning and E. coli culture results of samples collecte...Continue Reading

References

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Jan 30, 2009·Environmental Science & Technology·Richard L Whitman, Meredith B Nevers
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Jul 6, 2016·Journal of Environmental Quality·Julia DerxAlfred Paul Blaschke

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Citations

Oct 3, 2018·Journal of Environmental Quality·Asli AslanAsheley Chapman
Oct 3, 2018·Journal of Environmental Quality·Muruleedhara N ByappanahalliChristopher Otto
Mar 19, 2020·International Journal of Environmental Research and Public Health·Samuel DorevitchIra Heimler
Jun 17, 2020·Current Environmental Health Reports·David A Holcomb, Jill R Stewart
Oct 6, 2020·Journal of Environmental Quality·Mohammad Madani, Rajesh Seth
Mar 27, 2021·Journal of Microbiological Methods·Chad CrainMarva Seifert

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