Monobodies as enabling tools for structural and mechanistic biology.

Current Opinion in Structural Biology
Oliver HantschelShohei Koide

Abstract

Monobodies, built with the scaffold of the fibronectin type III domain, are among the most well-established synthetic binding proteins. They promote crystallization of challenging molecular systems. They have strong tendency to bind to functional sites and thus serve as drug-like molecules that perturb the biological functions of their targets. Monobodies lack disulfide bonds and thus they are particularly suited as genetically encoded reagents to be used intracellularly. This article reviews recent monobody-enabled studies that reveal new structures, molecular mechanisms and potential therapeutic opportunities. A systematic analysis of the crystal structures of monobody-target complexes suggests important attributes that make monobodies effective crystallization chaperones.

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Citations

Aug 15, 2020·Nature Communications·César Carrasco-LópezJosé L Avalos
Dec 20, 2020·The FEBS Journal·Ali A Kermani
Apr 2, 2020·Current Opinion in Structural Biology·Jan Steyaert, Todd O Yeates
Jun 23, 2021·Nature Communications·Georgios N HatzopoulosPierre Gönczy

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