Monocarboxylate transporter 1 gene expression in the ovine gastrointestinal tract

The Veterinary Journal
Doaa KiratS Kato

Abstract

In this study, we investigated the tissue distribution and expression of monocarboxylate transporter 1 (MCT1) along the gastrointestinal tract of sheep. Western blot analysis suggested the presence of MCT1 as a 43-kDa protein in immunoblots of membranes from the various tissues examined. The results of Western blotting were further confirmed by immunohistochemical studies, which revealed intense immunoreactivity for the MCT1 protein in the forestomach (rumen, reticulum and omasum) and large intestine (caecum, proximal and distal colon). Moderate reactivity, however, was detected in the abomasum, while no immunoreactivity could be seen in any regions of the small intestine examined. Furthermore, MCT1 was expressed at the mRNA level as determined by reverse transcriptase polymerase chain reaction (RT-PCR), which showed a band of the expected size (300 bp) in all tissues examined. From these results we concluded that MCT1 protein is highly expressed and distributed in the stomach and large intestine of sheep suggesting that MCT1 may play a significant role in the transport of short chain fatty acids and their metabolites in the gastrointestinal tract of ruminants.

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Citations

Jan 14, 2010·Journal of Biomedicine & Biotechnology·Judith KuzinskiMonika Schweigel
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·E E ConnorC Li
Jan 17, 2013·The British Journal of Nutrition·Virginie AlexandreAnne-Marie Davila
Jan 25, 2013·Digestive Diseases and Sciences·Ramesh KekudaUma Sundaram
Mar 20, 2016·Biochimica Et Biophysica Acta·Jhudit Pérez-EscuredoPierre Sonveaux
Jul 6, 2007·The Journal of Veterinary Medical Science·Yumiko ShimoyamaHiroyuki Taniyama
Mar 4, 2008·Cell Biology International·Harald Welter, Rolf Claus
Feb 26, 2013·Journal of Animal Physiology and Animal Nutrition·H PfannkucheG Gäbel
Sep 17, 2016·Cell Biology International·Hashemeya Al-MosauwiGavin Stewart
Apr 8, 2020·Asian Journal of Pharmaceutical Sciences·Gang WangYang Wang

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