Monocarboxylate transporters in subsarcolemmal and intermyofibrillar mitochondria

Biochemical and Biophysical Research Communications
Carley R BentonA Bonen

Abstract

Whether subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria contain monocarboxylate transporters (MCTs) is controversial. We have examined the presence of MCT1, 2, and 4 in highly purified SS and IMF mitochondria. These mitochondria were not contaminated with plasma membrane, sarcoplasmic reticulum or endosomal compartments, as the marker proteins for these sub-cellular compartments (Na+-K+-ATPase, Ca2+-ATPase, and the transferrin receptor) were not present in SS or IMF mitochondria. MCT1, MCT2, and MCT4 were all present at the plasma membrane. However, MCT1 and MCT4 were associated with SS mitochondria. In contrast, the IMF mitochondria were completely devoid of MCT1 and MCT4. However, MCT2 was associated with both SS and IMF mitochondria. These observations suggest that SS and IMF mitochondria have different capacities for metabolizing monocarboxylates. Thus, the controversy as to whether mitochondria can take up and oxidize lactate will need to take account of the different distribution of MCTs between SS and IMF mitochondria.

References

Jan 27, 1995·The Journal of Biological Chemistry·C K GarciaJ L Goldstein
Mar 24, 1998·Annual Review of Medicine·L J Goodyear, B B Kahn
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·L PellerinP J Magistretti
Oct 21, 1998·Developmental Neuroscience·L PellerinP J Magistretti
Oct 24, 1998·The Journal of Biological Chemistry·R Y LinD W Golde
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G A BrooksC E Butz
May 9, 2000·The Journal of Biological Chemistry·A BonenN N Tandon
Apr 6, 2002·American Journal of Physiology. Endocrinology and Metabolism·Mio TonouchiArend Bonen
Jun 4, 2002·The Journal of Physiology·George A Brooks
Jun 4, 2002·The Journal of Physiology·Kent SahlinMichail Tonkonogi
Dec 21, 2002·Acta Physiologica Scandinavica·K SepponenA R Pösö
May 10, 2003·Pflügers Archiv : European journal of physiology·Andrew P Halestrap, David Meredith
Jul 31, 2003·The Biochemical Journal·John C W Hildyard, Andrew P Halestrap
Nov 7, 2003·American Journal of Physiology. Cell Physiology·Jeffrey I MesserWayne T Willis
Apr 27, 2004·Journal of Applied Physiology·Yuko YoshidaArend Bonen
May 4, 2004·Journal of Applied Physiology·C Eric ButzGeorge A Brooks

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Citations

Mar 26, 2010·The Journal of Membrane Biology·Leslie E StefanykPaul J LeBlanc
Nov 26, 2008·European Journal of Applied Physiology·Yolande X R HarleyMalcolm Collins
Oct 2, 2013·The Journal of Physiological Sciences : JPS·Yu KitaokaHideo Hatta
Dec 22, 2012·Neuro-oncology·Vera Miranda-GonçalvesFátima Baltazar
May 18, 2012·TheScientificWorldJournal·Rogério Santos de Oliveira CruzFabrizio Caputo
Sep 9, 2010·Biological & Pharmaceutical Bulletin·Katsuya NarumiKen Iseki
Apr 2, 2008·Medicine and Science in Sports and Exercise·Takeshi Hashimoto, George A Brooks
Jan 11, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Jason C L Brown, James F Staples
Jun 4, 2005·The Journal of Physiology·Takeshi HashimotoGeorge A Brooks
May 11, 2010·Journal of Biomedicine & Biotechnology·Céline PinheiroFátima Baltazar
May 4, 2010·Research in Veterinary Science·A K MykkänenB Essén-Gustavsson
Oct 15, 2007·The Journal of Physiology·Arend BonenYuko Yoshida
May 27, 2011·Equine Veterinary Journal. Supplement·T RevoldB Essén-Gustavsson
Sep 30, 2014·Molecular Carcinogenesis·Julieta AfonsoFátima Baltazar
Feb 3, 2015·Journal of Cellular and Molecular Medicine·Isabel ValençaDaniela Ribeiro
Mar 20, 2012·International Journal of Pharmaceutics·Katsuya NarumiKen Iseki
Apr 11, 2006·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Arend BonenHideo Hatta
Sep 11, 2014·World Journal of Gastroenterology : WJG·Venâncio A AlvesFátima Baltazar
Oct 15, 2009·American Journal of Physiology. Endocrinology and Metabolism·Helena AkerudEva Wiberg-Itzel
Nov 11, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daisuke HoshinoArend Bonen
Oct 21, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Claire ThomasGeorge A Brooks

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