Monochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma

Molecular Cancer Research : MCR
Josephine M Y KoMaria Li Lung

Abstract

Loss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved los...Continue Reading

References

Feb 20, 1997·International Journal of Cancer. Journal International Du Cancer·K TamuraT Sekiya
Sep 25, 1999·International Journal of Cancer. Journal International Du Cancer·Y S TsangD P Huang
Nov 24, 1999·International Journal of Cancer. Journal International Du Cancer·A zur HausenH E Gabbert
Jul 4, 2001·Genes, Chromosomes & Cancer·G LiM R Emmert-Buck
Sep 1, 2001·Trends in Molecular Medicine·F de FraipontE G Van Meir
Jul 27, 2002·Cancer Letters·Xiao Ping HuangMing Rong Wang
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
Feb 13, 2004·International Journal of Cancer. Journal International Du Cancer·Yue ChengMaria Li Lung
Aug 1, 2006·Nature Genetics·Michael DewsAndrei Thomas-Tikhonenko
Jan 30, 2007·BMC Cancer·Maria Kost-AlimovaStefan Imreh

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Citations

Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Arthur Kwok Leung CheungMaria Li Lung
May 11, 2012·Chinese Journal of Cancer·Ji-Sheng LiLi-Li Li
Oct 18, 2015·European Journal of Medical Genetics·Benjamin KamienHimanshu Goel
Sep 30, 2010·International Journal of Cancer. Journal International Du Cancer·Stephen Ho Kin ChanMaria Li Lung
Dec 18, 2010·International Journal of Cancer. Journal International Du Cancer·King Chi ChanMaria Li Lung
Sep 10, 2014·International Journal of Cancer. Journal International Du Cancer·Xuesong YangMaria Li Lung
Feb 10, 2016·International Journal of Cancer. Journal International Du Cancer·Hoi Yan NgMaria Li Lung
Sep 6, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Abdullah AlholleFarida Latif
Jun 6, 2012·International Journal of Oncology·Ahmed KhamasKenichi Sugihara
Jun 4, 2017·Biochemical and Biophysical Research Communications·Zhen XuDong H Kim

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