Monoclonal anti-dipeptide antibodies cross-react with detyrosinated and glutamylated forms of tubulins

Cell Motility and the Cytoskeleton
R KuriyamaM Kimble

Abstract

Two monoclonal antibodies, GLU-1 and A1.6, raised against gamma-L-glutamyl-L-glutamic acid dipeptide (Glu-Glu) and Ca(2+)-dependent ATPase from Paramecium, respectively, recognized the dipeptide Glu-Glu sequence. Whereas the antibodies immunofluorescently stained very few, if any, cytoskeletal fibers in cultured mammalian cells, almost all interphase as well as mitotic spindle microtubules became visible after treatment of cells with carboxypeptidase A. Immunoblot analysis demonstrated intense cross-reaction of the antibodies to the alpha-tubulin subunit. alpha-Tubulin isotypes produced as fusion proteins in bacteria were labeled by both the antibodies only when the proteins did not contain a tyrosine residue at the C terminus, indicating that GLU-1 and A1.6 specifically recognize the detyrosinated form of alpha-tubulin. When microtubule protein purified from brain was probed, not only alpha-but also, to a lesser extent, beta-tubulin were revealed by the dipeptide antibodies. A synthetic tripeptide YED containing one glutamyl group linked to the second residue of the peptide via the gamma position was also recognized by the antibodies. Since this peptide sequence corresponds to the amino acid sequence of polyglutamyated class I...Continue Reading

References

Aug 4, 1975·Biochemical and Biophysical Research Communications·D Raybin, M Flavin
Feb 1, 1992·Molecular and Biochemical Parasitology·T G GearyR D Klein
Jun 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J C Bulinski, G G Gundersen
Jan 5, 1990·Science·B EddéP Denoulet
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J E AlexanderA Frankfurter
Mar 9, 1990·Cell·D W Cleveland
Mar 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J E MadlA J Beitz
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LeDizet, G Piperno
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W Steffen, R W Linck
Nov 1, 1987·The Journal of Cell Biology·E SchulzeM Kirschner
Mar 1, 1986·The Journal of Cell Biology·G G Gundersen, J C Bulinski
Jun 1, 1982·The Journal of Cell Biology·J V KilmartinC Milstein
Jan 1, 1994·Cell Motility and the Cytoskeleton·M H BréA Fleury
May 1, 1993·International Journal for Parasitology·M H Roos, W N Grant

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.