Monoclonal anti-galactan IgA J 539 binds intercatenarily to its polysaccharide antigen. Observations on the binding of antibody to a macromolecular antigen

Molecular Immunology
C P GlaudemansB N Manjula

Abstract

Highly purified P. Zopfii galactan of mol. wt 2 X 10(5) binds monoclonal IgA J 539 with a Ka of 5.80 X 10(5) M-1 if the polysaccharide concn is expressed in blocks of 30 galactosyl residues. This is the same Ka as found for the antibody and methyl beta (1,6)-beta-D-galactopyranosyltetraoside, the ligand capable of filling the entire combining area of the immunoglobulin. This same polysaccharide precipitates monomeric IgA J 539 on agar-double diffusion. It is concluded that the antibody binds to intercatenary galactosyl residues of the antigen.

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B N ManjulaM Potter
Jul 1, 1973·Carbohydrate Research·D J MannersJ F Ryley
Oct 10, 1973·Nature: New Biology·E A PadlanM Potter
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R J PoljakF Saul
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S RudikoffD R Davies
Nov 1, 1983·The Journal of Experimental Medicine·S RudikoffM Potter
Mar 1, 1983·Molecular Immunology·G EkborgC P Glaudemans

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Citations

Aug 1, 1986·Molecular Immunology·C P Glaudemans
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M KelleherE Handman
Aug 31, 2013·Glycoconjugate Journal·Eleonora AltmanBlair Harrison
Jun 3, 2015·Glycobiology·Omid Haji-GhassemiStephen V Evans
Jul 1, 1989·Journal of Molecular Recognition : JMR·G W BruseA A Lindberg
Jul 15, 1989·Carbohydrate Research·C P GlaudemansA S Rao

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