PMID: 6538484Apr 2, 1984Paper

Monoclonal anti-glycoprotein antibody that blocks cell adhesion in Polysphondylium pallidum

European Journal of Biochemistry
K TodaG Gerisch

Abstract

Polyclonal and monoclonal antibodies were prepared against a glycoprotein (gp 64) of Polysphondylium pallidum previously shown to act as a target site of adhesion-blocking Fab prepared from antisera against whole membranes of aggregation-competent cells. The purified glycoprotein, with a nominal Mr of 64000, could be fractionated into two subspecies, gp 64I and gp 64II, with apparent Mr of 66000 and 60000, as determined in 7.5% sodium dodecyl sulfate/polyacrylamide gels. Rabbit antibodies against purified gp 64 reacted not only with the two subspecies but also with many other membrane proteins. Almost all the cross-reactivity could be abolished by absorption of the antibodies with extensively purified gp 64. All monoclonal antibodies obtained by screening with gp 64 showed similar cross-reactivity. One monoclonal antibody specifically precipitating gp 64 was selected by screening with antigen that had been pretreated with anhydrous hydrogen fluoride for removal of carbohydrates. Fab from polyclonal anti-(gp 64) sera as well as one monoclonal Fab completely blocked cell adhesion of aggregation-competent P. pallidum cells. A carbohydrate fraction prepared by treatment of gp 64 with proteases and hydrazine completely neutralized t...Continue Reading

References

Oct 1, 1977·Analytical Biochemistry·A J Mort, D T Lamport
Apr 5, 1978·Journal of Molecular Biology·S Bozzaro, G Gerisch
Nov 1, 1972·Journal of Immunological Methods·H Beug, G Gerisch
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W R Springer, S H Barondes
Jan 1, 1980·Current Topics in Developmental Biology·G Gerisch
Feb 4, 1983·Science·G M Edelman
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·O ShimomuraJ T Bonner

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Citations

May 1, 1985·Developmental Biology·W R Springer, S H Barondes
Mar 1, 1986·Cell Differentiation·K TodaG Gerisch
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J S GillM Dworkin
Aug 1, 1985·Cell Differentiation·S Bozzaro

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