PMID: 6976413Jan 1, 1982Paper

Monoclonal anti-IgG autoantibodies derived from lipopolysaccharide-activated spleen cells of 129/Sv mice

The Journal of Experimental Medicine
J L Van Snick, P G Coulie

Abstract

In some colonies, 129/Sv mice produce, upon aging, a rheumatoid factor (RF) that is specific for mouse IgG2a but fails to react with IgG2a of the b allotype. It is not known whether this narrow specificity is due to the absence of other RF specificities in the repertoire of these mice or to the selective activation of the production of anti-IgG2a autoantibodies by a specific stimulus. To analyze the RF repertoire of 129/Sv mice, we have derived hybridomas from their spleen cells 3 d after an intraperitoneal injection of lipopolysaccharide. We have obtained 68 hybridomas secreting a monoclonal IgM with RF activity. This represents approximately 3 percent of the total number of hybridomas generated in four hybridizations. In addition, one monoclonal IgA RF was derived from unstimulated 129/Sv spleen cells. The specificities of these monoclonal RF were examined by testing their ability to bind to a panel of homologous and heterologous IgG preparations. The majority of the IgM RF reacted exclusively with a single mouse IgG subclass: 58 with IgG1, and 1 with IgG2a. Eight bound preferentially to IgG1 but cross-reacted to some extent with IgG2a and one was specific for a determinant shared by IgG1, IgG2a, and IgG3. The IgA RF derived ...Continue Reading

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Citations

Sep 1, 1989·Journal of Immunological Methods·C L Cambiaso, J N Limet
Dec 1, 1990·Molecular Immunology·E RajnavölgyiJ Gergely
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·G Dubray, J Limet
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Feb 6, 2007·Seminars in Immunology·Sean R Christensen, Mark J Shlomchik
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Aug 13, 2015·Evolutionary Anthropology·Omer Nevo, Eckhard W Heymann

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