Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain

Matrix Biology : Journal of the International Society for Matrix Biology
Susanne SchéelePeter Ekblom

Abstract

We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha1 chain. AL-4 has also been marketed as an alpha1 chain specific probe, but we show here that AL-4 detects mouse laminin beta1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta1 chain.

References

Jul 1, 1987·European Journal of Biochemistry·M PaulssonJ Engel
Feb 12, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L M SorokinP Ekblom
Dec 23, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M FalkP Ekblom
Jun 24, 2004·Human Molecular Genetics·Kinga GawlikMadeleine Durbeej
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susanne SchéelePeter Ekblom
Feb 8, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Joseph B MascarenhasJörg Stetefeld
Jun 28, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Monique AumailleyPeter D Yurchenco

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