PMID: 19912826Oct 1, 1991Paper

Monoclonal antibodies against a dopamine-releasing protein (DARP) arrest fetal development, decrease brain catecholamines, and increase adrenal weight of neonatal rats

Molecular and Cellular Neurosciences
S KuhananthanV D Ramirez

Abstract

Monoclonal antibodies (MAb) were produced against a partially purified dopamine-releasing protein (DARP) to examine the feasible physiological role of this factor during the development of the rat. These IgM isotype MAbs, and not other IgM antibodies (controls), induced a remarkable increase in fetal resorption and stillbirth rate and impaired the developmental increase of catecholamine concentration in the corpus striatum and hypothalamus of newborn rats. Neonatal injections of the anti-DARP MAb (a single injection of 200 mug, 24 h after birth or 40 mug on alternate days during the first 10 days) decreased the dopamine (DA) concentration of the corpus striatum by 30% on Day 10 and 15% on Day 25 and drastically impaired (by 43% on Day 25) the developmental increase in hypothalamic DA. Furthermore, in the hypothalamus, there was a marked decrease in epinephrine and an increase in norepinephrine (NE) concentration, suggesting an impairment in PNMT function. Neonatal anti-DARP injections also resulted in increased adrenal weight (45 and 44% on Days 10 and 25, respectively) and elevated NE content (anti-DARP, 315 +/- 12 ng, vs control, 223 +/- 16 ng, n = 6). Intrafetal injection of anti-DARP MAb (40 mug) on E 17 resulted in increas...Continue Reading

References

Jan 1, 1987·Annals of the New York Academy of Sciences·M T JonesA Di Sciullo
Jan 1, 1985·The Journal of Physiology·F J Seidler, T A Slotkin
Jan 1, 1987·Annals of the New York Academy of Sciences·M A HolzwarthN Kleitman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R S MorrisonR A Bradshaw
Apr 1, 1986·Journal of Neurochemistry·J LakshmananD A Fisher
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Kligman, D R Marshak
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J F MillD N Ishii
Aug 1, 1981·Neuroendocrinology·M Palkovits
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H N Antoniades

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