Abstract
Monoclonal antibodies against the lipooligosaccharide of Haemophilus ducreyi were produced. Two of them, MAHD6 and MAHD7, were found to be relatively, although not absolutely, specific and reacted with nearly all strains of Haemophilus ducreyi tested: 59 of 60 and 60 of 60, respectively. The diagnostic usefulness of MAHD7 was assessed. Clinical specimens collected in Zambia from patients with genital ulcers were tested using indirect immunofluorescence (IF), enzyme immunoassay (EIA), the polymerase chain reaction (PCR) and bacterial culture. Compared with culture, IF had a sensitivity of 100%; compared with PCR, sensitivity was 89%. The corresponding figures for the EIA were 83% and 74%, respectively. The sensitivity of culture compared with PCR was 63%. The results suggest that IF on genital smears using MAHD7 might be an excellent tool for the diagnosis of chancroid in high-prevalence populations. However, further evaluation of the specificity of this test is needed.
References
May 12, 1992·Biochemistry·N J PhillipsB W Gibson
May 25, 1990·Journal of Immunological Methods·J M LukA A Lindberg
Oct 1, 1990·Sexually Transmitted Diseases·Y DangorH J Koornhof
Aug 1, 1989·AIDS·A S LatifE Marowa
Apr 1, 1989·Clinical Microbiology Reviews·S A Morse
Dec 1, 1989·Genitourinary Medicine·Q N KarimR C Ballard
Sep 1, 1989·The Journal of Infectious Diseases·J K KreissL Corey
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·J M HorowitzE Harlow
Feb 1, 1988·AIDS·R M GreenblattA R Ronald
Dec 1, 1986·European Journal of Clinical Microbiology·D Y KunimotoA R Ronald
May 1, 1986·The Journal of Infectious Diseases·W O SchallaS A Morse
Nov 1, 1983·Journal of Clinical Microbiology·N I Carlin, A A Lindberg
Apr 1, 1984·Infection and Immunity·E J Hansen, T A Loftus
Apr 1, 1984·Sexually Transmitted Diseases·M V FastA R Ronald
Dec 1, 1981·The British Journal of Venereal Diseases·H NsanzeA Ronald
Apr 1, 1983·Carbohydrate Research·J H BanoubD H Shaw
Dec 1, 1994·Infection and Immunity·P A TottenW E Stamm
Mar 1, 1993·Journal of Clinical Microbiology·L ChuiR Marusyk
Citations
Sep 21, 2002·AIDS Patient Care and STDs·D A Lewis
Feb 5, 2003·Sexually Transmitted Diseases·Hinda J AhmedTeresa Lagergard
Jul 23, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Frisk, T Lagergård
Apr 6, 2005·The Journal of the Acoustical Society of America·Nathalie HenrichMichèle Castellengo
Jan 5, 2006·Journal of Clinical Microbiology·J MbwanaT Lagergård
Jun 20, 2000·Sexually Transmitted Infections·D A Lewis
Aug 26, 1998·Microbial Pathogenesis·A FriskT Lagergård
Apr 29, 2009·Microbes and Infection·Annika LundqvistTeresa Lagergård
Nov 26, 2010·International Journal of STD & AIDS·F MånssonM Unemo
Mar 6, 1998·Infection and Immunity·A FriskT Lagergård
May 14, 1998·Microbiology·X X GuE Van Dyck
Jan 18, 2002·Infection and Immunity·Hinda J AhmedTeresa Lagergård
Aug 1, 1997·Infection and Immunity·H J AhmedT Lagergård
Dec 2, 2000·Journal of Clinical Microbiology·M D HowardT J Inzana