PMID: 6171307Nov 18, 1981Paper

Monoclonal antibodies against human acid alpha-glucosidase

Biochimica Et Biophysica Acta
J HilkensJ Hilgers

Abstract

Acid alpha-glucosidase purified from human placenta was used to immunize a mouse (strain Balb/cHeA) according to a procedure described earlier (Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J. and Häring, P. (1980) J. Immunol. Methods 32, 297-304). After fusion of spleen cells with myeloma cells, about 10% of the hybrid clones obtained produced antibodies against acid alpha-glucosidase. Finally, eight stable clones producing antibodies against the enzyme were obtained. When purified acid alpha-glucosidase is analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, two major protein bands (mol. wt. 76000 and 70000,) a minor band of mol. wt. 9600 and several minor bands with a mol. wt. of 67000 or lower are seen. Since all these components react with the monoclonal antibodies, they must have at least one antigenic determinant in common.

References

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Citations

Jul 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·A M BesançonJ C Dreyfus
Jun 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N N ChuangB C Yang
Jan 1, 1994·Neuromuscular Disorders : NMD·D G PalmerJ M Howell
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E I GinnsJ A Barranger
Dec 1, 1987·Archives of Biochemistry and Biophysics·A Tsuji, Y Suzuki
Jan 1, 1987·Clinical Immunology and Immunopathology·R N WeijersH Kruijswijk
Jun 1, 1984·Archives of Biochemistry and Biophysics·F MartiniukR Hirschhorn
Mar 15, 1984·European Journal of Biochemistry·R P Oude ElferinkJ M Tager
Aug 15, 1984·International Journal of Cancer. Journal International Du Cancer·J HilkensM van der Valk
Aug 15, 1983·European Journal of Biochemistry·R A BarneveldJ M Tager
Jan 1, 1988·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·D J ScholJ Hilgers
May 20, 1985·Biochimica Et Biophysica Acta·R D HenkelJ C Williams
Dec 4, 1985·Biochimica Et Biophysica Acta·A C de VriesL M van Golde

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