Abstract
Acid alpha-glucosidase purified from human placenta was used to immunize a mouse (strain Balb/cHeA) according to a procedure described earlier (Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J. and Häring, P. (1980) J. Immunol. Methods 32, 297-304). After fusion of spleen cells with myeloma cells, about 10% of the hybrid clones obtained produced antibodies against acid alpha-glucosidase. Finally, eight stable clones producing antibodies against the enzyme were obtained. When purified acid alpha-glucosidase is analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, two major protein bands (mol. wt. 76000 and 70000,) a minor band of mol. wt. 9600 and several minor bands with a mol. wt. of 67000 or lower are seen. Since all these components react with the monoclonal antibodies, they must have at least one antigenic determinant in common.
References
Jan 30, 1978·Archives of Biochemistry and Biophysics·A K MurrayD H Brown
Dec 1, 1978·The Journal of Clinical Investigation·N G BeratisK Hirschhorn
Feb 1, 1979·Virology·R C NowinskiW N Burnette
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 16, 1978·Nature·M ShulmanG Köhler
Aug 7, 1975·Nature·G Köhler, C Milstein
Jun 22, 1971·Biochemistry·G FairbanksD F Wallach
Aug 15, 1970·Nature·U K Laemmli
May 1, 1967·Journal of Chromatography·F Auricchio, V Sica
Apr 23, 2009·Biochemistry·Ajay PandeJayanti Pande
Citations
Jan 1, 1983·Human Genetics·R A BarneveldA J Reuser
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·H R ZenklusenP U Heitz
Sep 1, 1984·The Histochemical Journal·J M Van DongenH Galjaard
Sep 1, 1985·The Histochemical Journal·H J SipsA J Reuser
Jul 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·A M BesançonJ C Dreyfus
Oct 1, 1987·Molecular Immunology·M de BoerJ M Tager
Jun 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N N ChuangB C Yang
Jan 1, 1994·Neuromuscular Disorders : NMD·D G PalmerJ M Howell
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E I GinnsJ A Barranger
Jan 1, 1989·The Journal of Cell Biology·U EilersH P Hauri
Dec 1, 1987·Archives of Biochemistry and Biophysics·A Tsuji, Y Suzuki
Feb 1, 1985·Analytical Biochemistry·S Vora
Feb 1, 1984·Biochemical Medicine·S PirruccelloE I Ginns
Jan 1, 1987·Clinical Immunology and Immunopathology·R N WeijersH Kruijswijk
Jun 1, 1985·Biochemical Medicine·T B HakvoortJ M Tager
Jun 1, 1984·Archives of Biochemistry and Biophysics·F MartiniukR Hirschhorn
Mar 15, 1984·European Journal of Biochemistry·R P Oude ElferinkJ M Tager
Mar 15, 1984·European Journal of Biochemistry·R P Oude ElferinkJ M Tager
Aug 15, 1984·International Journal of Cancer. Journal International Du Cancer·J HilkensM van der Valk
Jan 1, 1985·Hybridoma·R D HenkelJ C Williams
Jan 1, 1985·Medicinal Research Reviews·R G Cotton
Aug 15, 1983·European Journal of Biochemistry·R A BarneveldJ M Tager
Nov 1, 1991·DNA and Cell Biology·F MartiniukR Hirschhorn
Jan 1, 1988·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·D J ScholJ Hilgers
May 20, 1985·Biochimica Et Biophysica Acta·R D HenkelJ C Williams
Dec 4, 1985·Biochimica Et Biophysica Acta·A C de VriesL M van Golde