PMID: 6170512Sep 1, 1981Paper

Monoclonal antibodies against human fibroblast interferon

European Journal of Biochemistry
H K HochkeppelJ Collins

Abstract

A stable hybridoma clone derived by infusion of mouse myeloma cells (cell line Fo) and spleen cells of immunized mice has been isolated which secretes monoclonal antibodies against human fibroblast interferon (interferon-beta). The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. It also inhibits the antiviral activity of human fibroblast interferon-beta from which the sugar moiety has been cleaved off by enzymatic treatment. The antibody is therefore probably directed against the protein moiety of the interferon molecules.

References

Feb 1, 1978·European Journal of Immunology·G KöhlerM J Shulman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Nov 7, 1985·Journal of Immunological Methods·P M Ndumbe, R J Levinsky
Dec 1, 1972·Antimicrobial Agents and Chemotherapy·E A Havell, J Vilcek
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T StaehelinS Pestka
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger

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