Monoclonal antibodies against porcine LFA-1: species cross-reactivity and functional effects of beta-subunit-specific antibodies.

Molecular Immunology
J E HildrethM D Pescovitz

Abstract

As a first step in developing a porcine model system to study the effect of LFA-1-specific monoclonal antibodies on allograft rejection, we have identified anti-human LFA-1 monoclonal antibodies that cross-react with porcine leukocytes and have used one of these antibodies against the LFA-1 beta-subunit to purify porcine LFA-1. Immunization of Balb/c mice with the purified antigen yielded eight monoclonal antibodies directed against the beta-subunit of porcine LFA-1. These antibodies immunoprecipitated pig proteins corresponding to the human alpha-subunits of LFA-1 (CD11a), Mac-1 (CD11b) and LeuM5 (CD11c), and their common beta-subunit (CD18). Under both reducing and non-reducing conditions, the porcine beta-subunit showed a different electrophoretic mobility from that of the human beta-subunit. In addition, results of peptide mapping studies revealed structural differences between the beta-subunits of human and porcine LFA-1. The anti-LFA-1 beta-chain antibodies were found to cross-react with antigens expressed on rat, mouse, hamster, rabbit, dog, cow and human leukocytes, indicating that the beta-subunit contains highly conserved epitopes. Competitive binding studies showed that these antibodies and three anti-human LFA-1 ant...Continue Reading

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