Monoclonal antibodies against surface antigens of laryngeal carcinoma cells (author's transl)

Archives of Oto-rhino-laryngology
H P Zenner

Abstract

Humoral and cellular immune responses to laryngeal carcinomas suggest the presence of tumor-associated antigens on the surface of larynx carcinoma cells. Production of antisera against specific antigens on human tumors or tissues is complicated by the concomitant production of antibodies that react with all human cells. To circumvent this problem monospecific antibodies are needed. After isolation of larynx carcinoma cells by cell culture techniques outer cell membranes were prepared and used to immunize mice. From these mice the antibody-producing spleen cells were isolated and fused with myeloma cells resulting in antibody-secreting hybrid cells. Hybrid cells were cloned, thus forming monoclonal antibodies. A hybridoma clone was isolated producing antibodies that did not bind to various normal human cells lines, fibrocytes or lymphocytes, nor to cells of non-human origin. In contrast, the monoclonal antibody was able to identify various malignant target cells, including two laryngeal carcinomas and two salivary gland carcinomas. Monoclonal antibodies are a useful tool for detection of tumor-specific antigens and differentiation antigens. They represent an approach toward identifying and isolating cell surface components.

References

Jan 1, 1979·Archives of Oto-rhino-laryngology·H P ZennerI F Herrmann
Apr 1, 1978·The Laryngoscope·E H AdelsteinB A Barrett
Jan 15, 1974·International Journal of Cancer. Journal International Du Cancer·M J SimonsG B De-Thé
Mar 1, 1966·Journal of Bacteriology·G Henle, W Henle
Jan 21, 1972·Nature·E Ruoslahti, M Seppälä
Jan 1, 1970·Progress in Experimental Tumor Research·F M Burnet
Sep 1, 1965·The Journal of Experimental Medicine·P Gold, S O Freedman
Nov 15, 1970·International Journal of Cancer. Journal International Du Cancer·F Martin, M S Martin
Jan 1, 1966·Annual Review of Microbiology·G Klein

❮ Previous
Next ❯

Citations

Jan 1, 1988·Acta Oto-laryngologica. Supplementum·H P Zenner, F X Brunner
Aug 15, 1985·International Journal of Cancer. Journal International Du Cancer·K BoeheimS D Bernal
Mar 1, 1986·The Annals of Otology, Rhinology, and Laryngology·H P Zenner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.