PMID: 9161634Apr 1, 1997Paper

Monoclonal antibodies as direct probes for human sperm acrosome reaction

American Journal of Reproductive Immunology : AJRI
S DorjeeC Y Lee

Abstract

To develop simple and rapid assay procedures for determining human sperm acrosome reaction under various experimental conditions. Specific monoclonal antibodies against human sperm acrosome were generated and utilized as probes for acrosome reaction assays by direct labelling of antibodies with fluorescein-isothiocyanate (FITC). Among the generated monoclonal antibodies, HS-63 was shown to react with antigens in the acrosome content of permeabilized acrosome-intact human sperm, but not with those of the live ones. HSA-10 was found to recognize antigens on the surface of sperm inner acrosome and to react with acrosome-reacted human sperm in suspension. Following a swim-up procedure, highly motile sperm were recovered and incubated in BWW medium at 37 degrees C for 18 h. The percentages of acrosome-reacted sperm were determined at various incubation times by using FITC-labeled HS-63 or HSA-10 as the indicators in 10 min direct immunofluorescent assay. With the FITC-labeled HS-63 probe, the percentage of positively stained human sperm fixed in methanol decreased significantly after 18 h of incubation from > 90 to 70-80%. In contrast, positively stained live human sperm by HSA-10 increased significantly from < 1% to 15-30%. A high ...Continue Reading

References

Jan 1, 1976·Journal of Immunological Methods·J W Goding
Aug 1, 1992·Molecular Reproduction and Development·M OkabeT Mimura
May 1, 1989·Journal of Andrology·C J De JongeL J Zaneveld
Feb 1, 1987·Human Reproduction·C E Stock, L R Fraser
May 1, 1986·Fertility and Sterility·T NagaeH Yanagimachi
Jun 1, 1985·Biology of Reproduction·D P WolfK B Bechtol
Oct 1, 1985·International Journal of Andrology·P J MallettL R Fraser
Jul 1, 1984·British Medical Bulletin·K Sikora
Feb 1, 1981·The Journal of Experimental Zoology·P Talbot, R S Chacon
Jul 1, 1984·Journal of Reproductive Immunology·C Y LeeA C Menge
Jul 1, 1982·Journal of Reproductive Immunology·C Y LeeA C Menge
Dec 1, 1993·Journal of Reproductive Immunology·C Y LeeE Hsu
Oct 1, 1995·Journal of Reproductive Immunology·T YoshikiC Y Lee

❮ Previous
Next ❯

Citations

Apr 4, 1998·American Journal of Reproductive Immunology : AJRI·C Y LeeS Dorjee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Autoimmune Hemolytic Anemia

Autoimmune hemolytic anemia (AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to an insufficient number of oxygen-carrying red blood cells in the circulation. Discover the latest research on AIHA here.