PMID: 6979662Mar 1, 1982Paper

Monoclonal antibodies as probes for estrogen receptor detection and characterization

Journal of Steroid Biochemistry
G L Greene, E V Jensen

Abstract

Splenic lymphocytes from a Lewis rat, immunized with purified estradiol-receptor complex of calf uterine nuclei, were fused with cells of three mouse myeloma lines to yield several monoclonal lines of hybridoma cells that secrete antiestrophilin antibodies, which, like the antiserum of the immunized rat, react specifically with estrophilin of calf tissues. In contrast, a Lewis rat, immunized with cytosol estradiol-receptor complex from MCF-7 human breast cancer cells after purification by a novel affinity chromatography technique, gave antiserum that crossreacts with receptor from mammalian as well as avian tissues. Monoclonal antibodies secreted by three hybridoma cell lines derived from this immunized rat showed interesting differences in cross reactivity. All recognized receptor from primate sources, two of the three monoclonal preparations recognized receptor from calf and rat uterus as well, but none reacted with estrophilin from hen oviduct. Thus, in addition to similarities, there appear to be immunocytochemical differences between estrogen receptors from mammalian and avian sources and between receptors from primate and non-primate tissues. These monoclonal antibody preparations, recognizing different determinants on th...Continue Reading

References

Aug 15, 1978·European Journal of Biochemistry·M V Govindan, C E Sekeris
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G L GreeneE V Jensen
Jan 1, 1979·Immunological Reviews·T J McKearnF P Stuart
Nov 1, 1979·The New England Journal of Medicine·E R DeSombreM B Lipsett
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G L GreeneE V Jensen
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H J Eisen
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G L GreeneE V Jensen

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Citations

Jun 1, 1987·Journal of Neurology·K P LeschP Thierauf
Jan 1, 1989·Journal of Cancer Research and Clinical Oncology·M A NavarroP Rosel
Jan 1, 1988·Archives of Gynecology and Obstetrics·A ScharlA Bolte
Jan 1, 1993·Journal of Cancer Research and Clinical Oncology·A VeringH G Bender
Jan 1, 1986·Breast Cancer Research and Treatment·G Di FronzoF Rilke
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·H PickartzH Stein
Jan 1, 1993·Archives of Gynecology and Obstetrics·W E SimonF Hölzel
Jan 1, 1984·Journal of Steroid Biochemistry·G L GreeneE V Jensen
Jul 3, 1992·European Journal of Obstetrics, Gynecology, and Reproductive Biology·J BalaschJ A Vanrell
Dec 10, 1990·The Journal of Steroid Biochemistry and Molecular Biology·M Bhattacharjee, E Ali
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·R I NicholsonR W Blamey
Apr 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·H Hata, H Kuramoto
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·W HuaG M Clinton
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A ShimadaL S Miller
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Liao, D Witte
Apr 28, 2004·Plastic and Reconstructive Surgery·Osamu ItoYuiro Hata
Jun 17, 1997·Annals of the New York Academy of Sciences·P Moutsatsou, C E Sekeris

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