PMID: 7519243Aug 1, 1994Paper

Monoclonal antibodies defining functional sites on the toxin superantigen staphylococcal enterotoxin B

The Journal of Experimental Medicine
A R HamadJ W Kappler

Abstract

Four monoclonal antibodies (mAbs) were produced binding to four nonoverlapping epitopes on the superantigen staphylococcal enterotoxin B (SEB). The mAbs were tested for their ability to detect SEB bound to major histocompatibility complex (MHC) class II, to inhibit SEB binding to MHC class II, to inhibit SEB stimulation of T cell hybridomas, to bind to various nonfunctional mutants of SEB, and to capture and present SEB and its mutants to T cells in the absence of MHC class II. We concluded that two mAbs, B344 and B327, bound to epitopes not required for superantigen function, one mAb, 2B33, blocked an MHC interaction site on SEB, and the fourth mAb, B87, blocked the T cell recognition site on SEB. Moreover, two mAbs (B344 and 2B33) were capable of presenting SEB, although much less efficiently than APC, to CD4- but not CD4+ T cell hybridomas. The results confirm the functional domains on SEB originally defined by mutation and show that MHC class II is not always an essential component of the superantigen ligand.

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Citations

Apr 1, 1996·The Journal of Experimental Medicine·S C HongC A Janeway
Mar 24, 2010·Infection and Immunity·Mulualem E TilahunRichard A Goldsby
Jul 21, 2005·Journal of Bacteriology·Ching Wen Tseng, George C Stewart
Dec 24, 2010·Journal of Immune Based Therapies and Vaccines·Brian DrozdowskiLuigi Grasso
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·S MüllerH Köhler
Apr 21, 1997·The Journal of Experimental Medicine·A R HamadJ W Kappler
Jun 15, 2007·Seminars in Immunology·Eric J SundbergRoy A Mariuzza
Sep 1, 2015·Journal of the American Society of Nephrology : JASN·Maria N MartinaAbdel Rahim A Hamad
Jun 7, 2005·Advanced Drug Delivery Reviews·Nicholas J Mantis
Jul 24, 2012·Toxins·Siu-Kei Chow, Arturo Casadevall
Dec 1, 2013·Microbiology Spectrum·Bettina C Fries, Avanish K Varshney

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