Abstract
Four monoclonal antibodies (mAbs) were produced binding to four nonoverlapping epitopes on the superantigen staphylococcal enterotoxin B (SEB). The mAbs were tested for their ability to detect SEB bound to major histocompatibility complex (MHC) class II, to inhibit SEB binding to MHC class II, to inhibit SEB stimulation of T cell hybridomas, to bind to various nonfunctional mutants of SEB, and to capture and present SEB and its mutants to T cells in the absence of MHC class II. We concluded that two mAbs, B344 and B327, bound to epitopes not required for superantigen function, one mAb, 2B33, blocked an MHC interaction site on SEB, and the fourth mAb, B87, blocked the T cell recognition site on SEB. Moreover, two mAbs (B344 and 2B33) were capable of presenting SEB, although much less efficiently than APC, to CD4- but not CD4+ T cell hybridomas. The results confirm the functional domains on SEB originally defined by mutation and show that MHC class II is not always an essential component of the superantigen ligand.
References
Feb 1, 1992·The Journal of Experimental Medicine·J W KapplerP Marrack
Oct 29, 1992·Nature·S SwaminathanM Sax
Jan 1, 1991·Annual Review of Immunology·A HermanA M Pullen
Sep 1, 1990·The Journal of Experimental Medicine·A HermanP Marrack
Sep 1, 1990·The Journal of Experimental Medicine·Y ChoiB L Kotzin
May 11, 1990·Science·P Marrack, J Kappler
Feb 1, 1990·The Journal of Experimental Medicine·P MarrackJ Kappler
Jan 13, 1989·Cell·J WhiteP Marrack
May 1, 1989·The Journal of Experimental Medicine·J W KapplerP Marrack
May 19, 1989·Science·J KapplerP Marrack
May 18, 1989·Nature·J D Fraser
May 1, 1988·The Journal of Experimental Medicine·B Fleischer, H Schrezenmeier
May 1, 1981·The Journal of Experimental Medicine·J W KapplerP Marrack
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Apr 1, 1981·The Journal of Infectious Diseases·P M SchlievertR D Nishimura
Citations
Jan 1, 1996·Arthritis and Rheumatism·C TsaiD A Fox
Sep 19, 1998·Immunology·C GarciaT J Rogers
Jan 15, 2011·The Journal of Biological Chemistry·Avanish K VarshneyBettina C Fries
May 1, 1997·Preparative Biochemistry & Biotechnology·G A Bohach
Apr 1, 1996·The Journal of Experimental Medicine·S C HongC A Janeway
Mar 24, 2010·Infection and Immunity·Mulualem E TilahunRichard A Goldsby
May 19, 2000·Infection and Immunity·L T PangA W Chow
Jul 21, 2005·Journal of Bacteriology·Ching Wen Tseng, George C Stewart
Jun 1, 1997·The Journal of Clinical Investigation·S W Dow, T A Potter
Dec 24, 2010·Journal of Immune Based Therapies and Vaccines·Brian DrozdowskiLuigi Grasso
Jun 12, 2014·MBio·Avanish K VarshneyBettina C Fries
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·S MüllerH Köhler
Apr 30, 1998·Immunological Investigations·K B Abdul-Majid, M Abedi-Valurgerdi
Apr 21, 1997·The Journal of Experimental Medicine·A R HamadJ W Kappler
Jun 15, 2007·Seminars in Immunology·Eric J SundbergRoy A Mariuzza
Aug 8, 2009·Clinical and Experimental Immunology·J L HayworthS M M Haeryfar
Apr 9, 2001·Journal of Molecular Biology·M C KiekeD M Kranz
Sep 1, 2015·Journal of the American Society of Nephrology : JASN·Maria N MartinaAbdel Rahim A Hamad
Jun 7, 2005·Advanced Drug Delivery Reviews·Nicholas J Mantis
Jan 16, 2014·MAbs·Tian XiaYajun Guo
Feb 3, 2007·The EMBO Journal·Beenu MozaEric J Sundberg
Jan 13, 2015·The Journal of Biological Chemistry·Kaushik DuttaBettina C Fries
Jul 24, 2012·Toxins·Siu-Kei Chow, Arturo Casadevall
Dec 1, 2013·Microbiology Spectrum·Bettina C Fries, Avanish K Varshney
Apr 1, 1996·Infection and Immunity·R ShimonkevitzM Matsumura
Aug 1, 1995·Infection and Immunity·W MahanaW Mourad
Mar 20, 1999·Infection and Immunity·W Mahana