Monoclonal antibodies defining immunogenic regions of pili from Bacteroides nodosus strains 198 (A1), 265 (H1) and 336 (F1)

Immunology and Cell Biology
D YoungD J Stewart

Abstract

A total of 17 monoclonal antibodies (MoAb) were used to analyse the antigenic structure of pilus protein from three serogroups of Bacteroides nodosus. The four MoAb which agglutinated pili were serogroup (and subgroup) specific, and the agglutinating epitope was present on the pili monomer and dependent on the intra-chain disulfide bond. Non-agglutinating MoAb identified two further non-linear and serogroup-restricted epitopes on strain 198 (A1) pili and two linear epitopes on 336 (F1) and 265 (H1) pili. Three MoAb cross-reacted with pili from six of the eight major serogroups and recognized an epitope in the N-terminal region of the molecule. This panel of MoAb has therefore identified at least four epitopes on pilus protein and will facilitate serotopic analyses of the immunogenicity of each epitope in sheep during vaccination against footrot.

References

Jul 1, 1986·Journal of Bacteriology·T C EllemanD J Stewart
Apr 1, 1983·Australian Veterinary Journal·K J FaheyB L Clark
Nov 1, 1983·Australian Veterinary Journal·P D ClaxtonJ R Egerton

Citations

Mar 1, 1997·Veterinary Microbiology·Richard WhittingtonE K Moses
Apr 17, 2014·Vaccine·O P DhungyelRichard Whittington
Oct 1, 1993·Immunology and Cell Biology·T J O'MearaH W Raadsma

Related Concepts

Metazoa
Antibodies, Bacterial
Monoclonal Antibodies
Antibody Specificity
Antigenic Specificity
Antigens, Bacterial
Bacteroides
Cross Reactions
Bacterial Pilus
Serotyping

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