Monoclonal antibodies for the S2 subunit of spike of SARS-CoV-1 cross-react with the newly-emerged SARS-CoV-2

Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin
Zhiqiang ZhengYee-Joo Tan

Abstract

BackgroundA novel coronavirus, SARS-CoV-2, which emerged at the end of 2019 and causes COVID-19, has resulted in worldwide human infections. While genetically distinct, SARS-CoV-1, the aetiological agent responsible for an outbreak of severe acute respiratory syndrome (SARS) in 2002-2003, utilises the same host cell receptor as SARS-CoV-2 for entry: angiotensin-converting enzyme 2 (ACE2). Parts of the SARS-CoV-1 spike glycoprotein (S protein), which interacts with ACE2, appear conserved in SARS-CoV-2.AimThe cross-reactivity with SARS-CoV-2 of monoclonal antibodies (mAbs) previously generated against the S protein of SARS-CoV-1 was assessed.MethodsThe SARS-CoV-2 S protein sequence was aligned to those of SARS-CoV-1, Middle East respiratory syndrome (MERS) and common-cold coronaviruses. Abilities of mAbs generated against SARS-CoV-1 S protein to bind SARS-CoV-2 or its S protein were tested with SARS-CoV-2 infected cells as well as cells expressing either the full length protein or a fragment of its S2 subunit. Quantitative ELISA was also performed to compare binding of mAbs to recombinant S protein.ResultsAn immunogenic domain in the S2 subunit of SARS-CoV-1 S protein is highly conserved in SARS-CoV-2 but not in MERS and human co...Continue Reading

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Citations

Sep 18, 2020·Expert Opinion on Therapeutic Targets·Shibo JiangLanying Du
Jan 8, 2021·The Analyst·Maria Ana Cristina Huergo, Nguyen Thi Kim Thanh
Feb 6, 2021·ACS Sensors·Guoqiang Liu, James F Rusling
Feb 27, 2021·PLoS Pathogens·Kuan-Ying A HuangAlain R Townsend
Mar 26, 2021·International Immunopharmacology·Maryam BayatReyhaneh Eftekhari
Sep 3, 2020·International Immunopharmacology·Hajar OwjiNasim Hajighahramani
May 14, 2021·Nature Structural & Molecular Biology·Maximilian M SauerDavid Veesler
Jun 3, 2021·Nature Reviews. Microbiology·William T HarveyDavid L Robertson
Jun 18, 2021·Frontiers in Molecular Biosciences·Arnaud John Kombe KombeTengchuan Jin
Jun 22, 2021·Frontiers in Medicine·Arjun SharmaMarkus Bosmann

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Datasets Mentioned

BETA
QHD43416.1
DQ168569
DQ168570
CR3022
MT093571

Methods Mentioned

BETA
ELISA
protein assay
transfection
electrophoresis
ELISAs

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